We narrowed to 14,168 results for: flag

1. pJL30

   Plasmid

   #174064

   Purpose

   Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.

   Depositor

   Albert Keung

   Article

   Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

   Insert

   pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   pGal1

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL15

   Plasmid

   #174069

   Purpose

   Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

   Depositor

   Albert Keung

   Article

   Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

   Insert

   pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   pGal1

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

   Plasmid

   #162957

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

   Plasmid

   #162955

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. DT040A

   Plasmid

   #159401

   Purpose

   Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.J23117/J23106-sfGFP
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   hybrid J23117-J23106 promoter

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

   Plasmid

   #149346

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   Cbc

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

   Plasmid

   #149347

   Purpose

   Mammalian expression vector for SpyCas9 and puromycin resistance

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

   Plasmid

   #149350

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM28

   Plasmid

   #154165

   Purpose

   Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel1A
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-FNLSHF1-P2A-Puro

    Plasmid

    #136901

    Purpose

    Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHF1
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    R661A,Q695A,Q926A

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS

    Plasmid

    #126203

    Purpose

    Mammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIC3Nme
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1149-pCSDest-AcrIIA4-mCherry-miR122BS

    Plasmid

    #126205

    Purpose

    Mammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIA4
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEX Tom20 mVenus BoPD

    Plasmid

    #114466

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoPD

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoPD
    

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFETCh_ZNF511

    Plasmid

    #104037

    Purpose

    Donor vector for 3' FLAG tag of human ZNF511

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF511 homology arms (ZNF511 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mammalian

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFETCh_RFXANK

    Plasmid

    #86296

    Purpose

    Donor vector for 3' FLAG tag of human RFXANK

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RFXANK homology arms (RFXANK Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. POLN delP_pDEST17

    Plasmid

    #64886

    Purpose

    bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail

    Depositor

    Richard Wood

    Article

    Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5

    Insert

    POLN (POLN Human)
    

    Tags

    Flag and His

    Expression

    Bacterial

    Mutation

    with a short truncation of the C-terminal proline…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAD-g6

    Plasmid

    #61497

    Purpose

    For expression of C. briggsae gld-1, used for rescue of C. elegans q485 homozygotes

    Depositor

    Eric Haag

    Article

    Beadell et al Proc Natl Acad Sci U S A. 2011 Dec 6;108

    Insert

    gld-1 (Cbr-gld-1 Caenorhabditis briggsae)
    

    Tags

    FLAG and His

    Expression

    Worm

    Promoter

    gld1

    Available Since

    Feb. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

18. TAL3589

    Plasmid

    #47308

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ybx1-right (ybx1 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. TAL3588

    Plasmid

    #47307

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ybx1-left (ybx1 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

20. TAL3527

    Plasmid

    #47246

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive)-right (rp2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only