We narrowed to 134 results for: spycatcher
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Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
Plasmid#117656PurposeBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.DepositorInsertssfCherry2(1-10)
sfCherry2(11)-SpyCatcher002
ExpressionBacterialPromoterT7Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
TwinStrepSUMO-Ad5-SpyCatcher
Plasmid#243751PurposeFor affinity purification of A5 knob fused to SpyCatcherDepositorInsertAd5-SpyCatcher
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher-mi3
Plasmid#112255PurposeExpresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertSpyCatcher-mi3
TagsC-tagExpressionBacterialPromoterT7Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21 MBPx-SpyCatcher
Plasmid#72327PurposeBacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.DepositorInsertpET21 MBPx-SpyCatcher
TagsN-terminal His6 tagExpressionBacterialMutationMBP contains A312V, I317V mutations and 172, 173,…Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFN18A-HaloTag-SpyCatcher
Plasmid#206041PurposeHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTagDepositorTypeEmpty backboneTagsHaloTag and SpyCatcher-8HisExpressionBacterialPromoterT7Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
TagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dead Streptavidin-SpyCatcher
Plasmid#59547PurposepET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramersDepositorInsertDead-dloop-Streptavidin-SpyCatcher
TagsN-terminally shortened SpyCatcherExpressionBacterialMutationPolyAsp in L3/4 loop for ion-exchangeAvailable SinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only