We narrowed to 128 results for: spycatcher

1. pDEST14-Cys-SpyCatcher002

   Plasmid

   #102829

   Purpose

   Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Cys-SpyCatcher002
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Cysteine residue in N-terminal region

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFN18A-HaloTag-SpyCatcher

   Plasmid

   #206041

   Purpose

   HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   HaloTag and SpyCatcher-8His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

   Plasmid

   #117656

   Purpose

   Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Inserts

   sfCherry2(1-10)
   sfCherry2(11)-SpyCatcher002
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21 MBPx-SpyCatcher

   Plasmid

   #72327

   Purpose

   Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET21 MBPx-SpyCatcher
   

   Use

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   MBP contains A312V, I317V mutations and 172, 173,…

   Promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-SpyCatcher002-MBP

   Plasmid

   #216295

   Purpose

   Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher002-MBP
   

   Use

   Tags

   His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIBA3-SpyCatcher-sfGFP

   Plasmid

   #107420

   Purpose

   For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   SpyCatcher
   

   Use

   Tags

   His tag, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-Avitag-SpyCatcher002

   Plasmid

   #102828

   Purpose

   Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Avitag-SpyCatcher002
   

   Use

   Tags

   Avitag, GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Use

   Tags

   C-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dead Streptavidin-SpyCatcher

   Plasmid

   #59547

   Purpose

   pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead-dloop-Streptavidin-SpyCatcher
   

   Use

   Tags

   N-terminally shortened SpyCatcher

   Expression

   Bacterial

   Mutation

   PolyAsp in L3/4 loop for ion-exchange

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Use

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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