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pcDNA5/FRT/TO C1-APEX2

Plasmid

#129641

Purpose

Express ER-localized C1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

C1(1-29)-APEX2-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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PB-PARP1-Apex2–eGFP

Plasmid

#211555

Purpose

A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

Apex2-eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKH159_FLAG-APEX2-YIPF4 WT

Plasmid

#214978

Purpose

Flag-APEX2 tagged YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF4 (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH161_FLAG-APEX2-YIPF3 WT

Plasmid

#214980

Purpose

Flag-APEX2 tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3 (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-dAPEX2

Plasmid

#117175

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRibo-Sec-APEX2m

Plasmid

#176844

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Use

Inducible

Tags

Expression

Bacterial

Mutation

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Tags

Expression

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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IF701: pMVP (L5-L4) APEX2

Plasmid

#121725

Purpose

pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-mCherry

Plasmid

#188944

Purpose

Middle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

mito-APEX2_p2A_APEX2-H2B_p2A_mCherry

Use

Middle entry vector for gateway (l1-l2)

Tags

Expression

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRibo-Sec-APEX2

Plasmid

#111699

Purpose

Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Use

Tags

V5

Expression

Bacterial

Mutation

Promoter

hsp60-ribo

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMV306-ribo-APEX2

Plasmid

#111700

Purpose

Theophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (integrating)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Use

Tags

V5

Expression

Bacterial

Mutation

Promoter

hsp60-ribo

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-C1(1-29)-EGFP-APEX2-V5

Plasmid

#236400

Purpose

Expresses a fluorescent Endoplasmic Reticulum-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

CYP2C1 fragment-EGFP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP Keima-PEX26

Plasmid

#224557

Purpose

expresses Keima fused PEX26 peroxisomal localization sequence in mammalian cells

Depositor

Gregory Wyant, William Kaelin

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

Keima-PEX26 (PEX26 Human)

Use

Retroviral

Tags

Keima

Expression

Mammalian

Mutation

PEX26 peroxisomal targeting sequence

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-APEX2

Plasmid

#170526

Purpose

Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Leishmania cell free expression clone

Tags

APEX2 and Lifeact

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Lifeact-mEos2-APEX2

Plasmid

#115563

Purpose

Lifeact-mEos2-APEX2 fusion for PALM and CLEM. Parton lab clone IET

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mEos2

Use

Tags

APEX2 and Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2 (GC rich)

Plasmid

#115550

Purpose

Multisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHS

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

APEX2 (GC rich)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Expression

Mutation

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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