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Showing: 161 - 200 of 253 results

  1. pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

    Plasmid
    #231353
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231353-plasmid-map-sequence-id-459833

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  2. pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

    Plasmid
    #231357
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231357-plasmid-map-sequence-id-459837

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  3. pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

    Plasmid
    #231358
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231358-plasmid-map-sequence-id-459844

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  4. pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

    Plasmid
    #231359
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231359-plasmid-map-sequence-id-459843

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  5. pRAV23

    Plasmid
    #67715
    Purpose
    Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pRAV24

    Plasmid
    #67716
    Purpose
    Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Use
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

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  9. pAIP4_3V46

    Plasmid
    #234165
    Purpose
    Heterologous protein expression of cell division control protein 73 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3V46 (CDC73 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234165-plasmid-map-sequence-id-464131

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  10. pRAV12

    Plasmid
    #67713
    Purpose
    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67713-plasmid-map-sequence-id-126588

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  12. pTmaM4

    Plasmid
    #67714
    Purpose
    Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Thermotoga marítima Ffh-M domain
    Use
    Tags
    hexahistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67714-plasmid-map-sequence-id-126589

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  15. pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

    Plasmid
    #231352
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231352-plasmid-map-sequence-id-459729

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  27. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99218-plasmid-map-sequence-id-192402

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  28. pDB023f

    Plasmid
    #99217
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99217-plasmid-map-sequence-id-192398

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  29. pDB023k

    Plasmid
    #99219
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12, 203, and 527 of T7 …
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99219-plasmid-map-sequence-id-192936

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  30. pHT-497

    Plasmid
    #87756
    Purpose
    Bacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)
    Depositor
    Yu-Wen Hwang
    Insert
    Gene coding for rat DYRK1A kinase domain (residues 1-497)
    Use
    Tags
    6X histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87756-plasmid-map-sequence-id-173678

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  31. pHT-PRD

    Plasmid
    #87755
    Purpose
    Bacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)
    Depositor
    Yu-Wen Hwang
    Insert
    Semi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
    Use
    Tags
    6X histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87755-plasmid-map-sequence-id-173677

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  32. T444T

    Plasmid
    #113081
    Purpose
    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113081-plasmid-map-sequence-id-248277

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  33. T777T

    Plasmid
    #113082
    Purpose
    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113082-plasmid-map-sequence-id-218958

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  36. pET-DEST42-dre4

    Plasmid
    #170440
    Purpose
    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.
    Depositor
    Joan Conaway
    Article
    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e
    Insert
    dre4 (dre4 Fly)
    Use
    Tags
    6xHis and V5
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-20
    Promoter
    T7
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170440-plasmid-map-sequence-id-338015

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  37. rFIXX-bp chain A (IRESneo)

    Plasmid
    #82450
    Purpose
    Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain A
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    82450-plasmid-map-sequence-id-156201

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  38. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    82451-plasmid-map-sequence-id-156993

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  39. pCS4

    Plasmid
    #55751
    Purpose
    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-59 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55751-plasmid-map-sequence-id-86408

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  40. pCS5

    Plasmid
    #55750
    Purpose
    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 59-264 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55750-plasmid-map-sequence-id-86407

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Showing: 161 - 200 of 253 results