We narrowed to 278 results for: T7 rna polymerase
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pSL1180-NiV L(2k)-mCherry/eGFP
Plasmid#234654PurposeMinigenome plasmid of NiV Bangladesh strainDepositorInsertspart L gene
mCherry
eGFP
UseIn cell lines expressing bacteriophage t7 rna pol…ExpressionMammalianAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7
Plasmid#231350PurposeSelf complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7
Plasmid#231356PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7
Plasmid#231348PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X
Plasmid#182365PurposeExpresses Neq2X DNA polymeraseDepositorInsertNeq2X
TagsHis-tagExpressionBacterialPromoterT7Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-TAQ_E602D
Plasmid#166944PurposeBacterial expression of Taq DNA polymeraseDepositorInsertThermus aquaticus DNA polymerase
Tags6X HisExpressionBacterialMutationE602DPromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7opt in pCAGGS
Plasmid#65974PurposeHuman codon-optimized T7 polymeraseDepositorInsertT7-opt
ExpressionMammalianAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIA1363
Plasmid#166861PurposeExpression of codon optimized SARS-COV-2 Nsp8 in E. coliDepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1362
Plasmid#166860PurposeExpression of codon optimized SARS-COV-2 Nsp7 in E. coliDepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7
Plasmid#231353PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7
Plasmid#231357PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7
Plasmid#231358PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7
Plasmid#231359PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
C98m
Plasmid#121002PurposeMoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertGp2 toxin
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pARiBo1
Plasmid#98892PurposeExpresses ARiBo-fused RNA in vitro with T7 RNA polymeraseDepositorInsertARiBo1
UseIn vitro transcription vectorPromoterT7 promoterAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15bLCFPT7
Plasmid#53545PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistantDepositorInsertT7 RNAP
ExpressionBacterialAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorTagsHis6ExpressionBacterialPromoterT7Available SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDH3pro-rtTA-tetO7pro-T7pol-pRS413
Plasmid#239293PurposeExpresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression systemDepositorInsertsT7 polymerase with NLS and P278L
reverse tetracycline transactivator (rtTA)
ExpressionYeastMutationContains M2-SE-G72P mutations, described in Addge…PromoterTDH3 and tetracycline-responsive element (TRE) an…Available SinceJune 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRAV24
Plasmid#67716PurposeParental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
ExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRAV23
Plasmid#67715PurposeParental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
ExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJ1
Plasmid#138379PurposepEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZDepositorTagsHis10ExpressionBacterialMutationClamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…PromoterT7Available SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNG1256
Plasmid#149710PurposeExpresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complexDepositorAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3V46
Plasmid#234165PurposeHeterologous protein expression of cell division control protein 73 in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRAV12
Plasmid#67713PurposeParental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
UseSynthetic BiologyExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHMM
Plasmid#67717PurposeExpression vector MBP/hexahistidine tagged variant of the MS2 coat proteinDepositorInsertHexahistidine/Maltose binding protein/MS2 coat protein fusion
TagsHexahistidine tagExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTmaM4
Plasmid#67714PurposeExpression vector hexahistidine tagged variant of the Thermotoga maritima M-domain proteinDepositorInsertThermotoga marítima Ffh-M domain
Tagshexahistidine tagExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7
Plasmid#231352PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.DepositorInsertT7-SP6-BC(p11-14)
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
CPH3566 (pDEST15_Spt6 1223-1451)
Plasmid#91814PurposeBacterial expression of residues 1223-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
TagsGSTExpressionBacterialMutationdeleted residues 1-1222PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH1141 (pET151_Spt6 1247-1451)
Plasmid#91815PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationdeleted residues 1-1246PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcB
Plasmid#51282PurposeE. coli expression vector for E. coli RtcB RNA ligaseDepositorInsertE. coli RtcB
Tags6xHisExpressionBacterialPromoterT7 RNA PolymeraseAvailable SinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2,0_3E5E_eGFP
Plasmid#69359PurposeEncodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoterDepositorInsert3E5E eGFP minigenome
UseT7PromoterT7Available SinceNov. 30, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CPH3567 (pET151_Spt6 1247-1451 R1282H)
Plasmid#91816PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged arginine 1282 to histidine; deleted resid…PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3495 (pET151_Spt6 1247-1451 K1435A)
Plasmid#91817PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged lysine 1435 to alanine; deleted residues …PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)
Plasmid#91818PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutationsDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged lysine 1355 and lysine 1435 to alanines; …PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTM1_NP_ZEBOV
Plasmid#69121PurposeEncodes for Ebola Virus Zaire NP under control of a T7 RNA polymerase promoterDepositorAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTM1_VP35_ZEBOV
Plasmid#68121PurposeEncodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.DepositorAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only
