We narrowed to 4,319 results for: U6 gRNA

1. pT4-U6-sgRNA-CMV-EGFP

   Plasmid

   #239587

   Purpose

   Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

   Depositor

   Andreas Moor

   Article

   Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

   Type

   Empty backbone

   Use

   Sleeping beauty transposone

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-AsCas12f-full-length-sgRNA

   Plasmid

   #204638

   Purpose

   Expression of full-length AsCas12f sgRNA in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   full-length AsCas12f sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-(BbsI)sgRNA_CAG-Venus-bpA

   Plasmid

   #86985

   Purpose

   For cloning and expression of sgRNA together with Venus expression

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Venus GFP variant

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-U6-sgRNA-CMV-puro

   Plasmid

   #169450

   Purpose

   backbone for sgRNA expression

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLb-Cpf1-pGL3-U6-sgRNA

   Plasmid

   #107682

   Purpose

   Expresses LbCpf1 crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-U6-gRNA-TnT-Cre

   Plasmid

   #132551

   Purpose

   For loss-of-function experiments in cardiomyocytes

   Depositor

   William Pu

   Inserts

   TnT-Cre
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TnT and U6

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

   Plasmid

   #118842

   Purpose

   Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   cdkn2a gRNA (LOC100329528 Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV U6-sgRNA UbC-tdTomato

   Plasmid

   #106949

   Purpose

   sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   sgRNA BsmBI/Esp3I cloning site
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MiniCoopR U6:gRNA p53, mitfa:Cas9

   Plasmid

   #118841

   Purpose

   Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   tp53 gRNA (tp53 Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sgRNA scaffold-spacer-human U6

    Plasmid

    #214697

    Purpose

    provide "sgRNA scaffold-spacer-human U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-human U6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAs-Cpf1-pGL3-U6-sgRNA

    Plasmid

    #107683

    Purpose

    Expresses AsCpf1 crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS23.U6<ActB.mus_C-term-KI_gRNA

    Plasmid

    #207408

    Purpose

    gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    ActB
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO1-puro-U6-sgRNA-TGM2

    Plasmid

    #69239

    Purpose

    U6 driven SpCas9 sgRNA expression for TGM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TGM2 sgRNA (TGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6-CB2gRNA-CBh-mCherry

    Plasmid

    #91948

    Purpose

    Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO1-puro-U6-sgRNA-LRTM2

    Plasmid

    #69237

    Purpose

    U6 driven SpCas9 sgRNA expression for LRTM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    LRTM2 sgRNA (LRTM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-U6-epegRNA-SV40-mCherry

    Plasmid

    #240538

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.

    Depositor

    Wensheng Wei

    Article

    Niu et al bioRxiv 2024.06.16.599253

    Insert

    epegRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-U6-sgRNA-EF1a-LibVec

    Plasmid

    #239591

    Purpose

    AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-U6-sgRNA-BFP-Puro

    Plasmid

    #229014

    Purpose

    Perturb Seq lentiviral sgRNA vector

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA scaffold-spacer-mouse U6

    Plasmid

    #214698

    Purpose

    provide "sgRNA scaffold-spacer-mouse U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-mouse U6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge