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Showing: 81 - 100 of 141943 results

  1. his3MX6-ins3

    Plasmid
    #195040
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    His5+
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195040-plasmid-map-sequence-id-384243

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  4. natMX-ins7

    Plasmid
    #195044
    Purpose
    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195044-plasmid-map-sequence-id-384248

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  6. CAPZB2-TwinStrepII

    Plasmid
    #83194
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CAPZB2 (Capzb Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83194-plasmid-map-sequence-id-163493

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  7. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid
    #197268
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-v2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197268-plasmid-map-sequence-id-389661

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  12. pIS1 INSIG2

    Plasmid
    #60798
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    INSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60798-plasmid-map-sequence-id-97809

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  16. pIS1 mINSIG2

    Plasmid
    #60799
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    INSIG2 3'UTR and mutated miR-155 binding site (INSIG2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    60799-plasmid-map-sequence-id-112562

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Showing: 81 - 100 of 141943 results