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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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LacI/109-pHG165c

Plasmid

#90058

Purpose

WT LacI polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI/109

Use

Tags

Expression

Bacterial

Mutation

109

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pXW109PmerT-Pecf11-gfp

Plasmid

#160822

Purpose

Two-layered mercury responsive circuit carrying an ECF11-based amplifier

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23109-32merR-t-PmerT-33ecf11-Pecf11-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PJ23109, PmerT, Pecf11

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrGAP R1090A

Plasmid

#38176

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)

Use

Tags

Xpress

Expression

Mammalian

Mutation

aa 1004-1271 with R1090A point mutation

Promoter

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Use

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-2T FRB R2109A

Plasmid

#26609

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

FRB domain only (aa 2015-2114); R2109A

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
CJ49 (cg11094 utr)

Plasmid

#17777

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg11094 utr fragment

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pXTK109 [Term_bla-v2_PartType10]

Plasmid

#229346

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Term_bla-v2_PartType10

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

bla-v2 Terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_GR-RE_MLPmin_BC1093-luc2

Plasmid

#227113

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC109-P2B1

Plasmid

#202950

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC109

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC109

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet Response Element (TRE)

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1093-1322_S

Plasmid

#147532

Purpose

Mammalian Expression of HsNot1iso1_1093-1322

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1093-1322 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E109_I112delinsD

Plasmid

#116468

Purpose

Lentiviral expression of PIK3CA E109_I112delinsD

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

E109_I112delinsD

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TESK1 gRNA (BRDN0001145109)

Plasmid

#77892

Purpose

3rd generation lentiviral gRNA plasmid targeting human TESK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TESK1 (TESK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM6 gRNA (BRDN0001148109)

Plasmid

#77336

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM6

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM6 (TRPM6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACB gRNA (BRDN0001147109)

Plasmid

#75961

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACB (PRKACB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKMT1A gRNA (BRDN0001149109)

Plasmid

#75880

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKMT1A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKMT1A,CKMT1B (CKMT1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p6109 pSG5-HPV31-E2(HA)

Plasmid

#24740

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 Feb 24. ():.

Insert

HPV31 E2 (HA)

Use

Tags

HA tag in hinge region

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D21A

Plasmid

#19073

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Use

Tags

C tag

Expression

Bacterial

Mutation

E18A/D21A

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D22A

Plasmid

#19078

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Use

Tags

C tag

Expression

Bacterial

Mutation

E18A/D22A

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only