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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Gs protein, alpha subunit [N192/12]

Plasmid

#190302

Purpose

Mammalian Expression Plasmid of anti-Gs protein, alpha subunit (Cattle). Derived from hybridoma N192/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Gs protein, alpha subunit (Bos taurus) recombinant Mouse monoclonal antibody (GNAS Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, V18D

Plasmid

#180612

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, V18D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, F98D

Plasmid

#180613

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, F98D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH

Plasmid

#148878

Purpose

Mammalian Expression of TNFalpha-ARE-polyA-Malat

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

TNFalpha-ARE-polyA-Malat (TNF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA

Plasmid

#183802

Purpose

AAV vector to express nuclear localized dsRed-Express from CaMKIIa promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

dsRed-Express

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA216-CoV2-RBD-alpha variant

Plasmid

#194599

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 alpha

Depositor

Ben Murrell

Insert

CoV2-RBD-alpha variant (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

Plasmid

#172105

Purpose

Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)-Gαs (GNAS Human)

Use

Tags

Expression

Mammalian

Mutation

Gαs: C3S

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha6 [N229A/32R]

Plasmid

#182109

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha6 (Mouse). Derived from hybridoma N229A/32.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Alpha6 (Mus musculus) recombinant mouse monoclonal antibody (Gabra6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

Plasmid

#170236

Purpose

Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CHUK (CHUK Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

S176E, S180E

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDUAL-Pnmt1-Ost4-GFP-MTalpha

Plasmid

#154370

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-1alpha

Plasmid

#124155

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-2alpha

Plasmid

#124159

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

Plasmid

#110159

Purpose

Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta with CaMKKalpha RP domain

Plasmid

#33332

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

CaMKKbeta RP domain (203-225) replaced with CaMKK…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha

Plasmid

#33330

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

CaMKKbeta N-terminus (1-164) was fused to body fo…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKalpha N-terminus on CaMKKbeta

Plasmid

#33329

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

CaMKKalpha N-terminus(1-128) was fused with CaMKK…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
Venus Cam KII alpha 1-315aa

Plasmid

#29431

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Use

Tags

Venus

Expression

Mammalian

Mutation

Expresses CamKIIa amino acids 1-315

Promoter

Available since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

Plasmid

#179719

Purpose

Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

Plasmid

#179723

Purpose

Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge