We narrowed to 1,413 results for: Flp

1. EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB

   Plasmid

   #236140

   Purpose

   Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   ZNF35(5-8)-ZNF250(1-4)-deImmunLink-ZIK1KRAB
   

   Expression

   Mammalian

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. EW425 CMV-TO-ZNF35(5-8)-IKZF1(2-3)-realDeImmLink-ZIK1KRAB

   Plasmid

   #236142

   Purpose

   Plasmid encoding the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   ZNF35(5-8)-IKZF1(2-3)-deImmunLink-ZIK1KRAB
   

   Expression

   Mammalian

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)

   Plasmid

   #233430

   Purpose

   LIDAR all components on single transcript (as shown in Figure 4A), using EMCV IRES to separate each component

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   sensor-IRESv0-LIDARst(IRESv0-AVPR2)
   

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM

   Plasmid

   #233429

   Purpose

   Positive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-LIDARsensor(W)-hIgG1-TM
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP

   Plasmid

   #233408

   Purpose

   LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xPP7))cSG-EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

   Plasmid

   #233415

   Purpose

   LIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. XZ400 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-TEVP (equivalent to EK0461)

   Plasmid

   #233414

   Purpose

   LIDAR reporter RNA; mCherry as marker, TEVP as output (equivalent to EK0461)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-TEVP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. APL0038 Ac5-mCherry-SGc(stem-1(3xMS2))cSG-EGFP

   Plasmid

   #233410

   Purpose

   AC5 promoter driven LIDAR reporter RNA for drosophila cell expression (mCherry as marker, EGFP as output)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Ac5

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)

   Plasmid

   #233378

   Purpose

   PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   FKBP-ADAR2dd
   

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   PGK

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-C+F-ArchT-GFP

    Plasmid

    #209700

    Purpose

    Expresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinases

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    ArchT-GFP
    

    Use

    AAV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

    Plasmid

    #205603

    Purpose

    Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

    Depositor

    Tarun Kapoor

    Article

    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

    Insert

    γ-tubulinWT-RNAi-resistant-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G2228A, C2231T, C2237T, G2240A

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-fDIO-tdTomato-WPRE

    Plasmid

    #187112

    Purpose

    FLP-dependent expression of tdTomato under EF1a promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-fDIO-mNeonGreen

    Plasmid

    #99133

    Purpose

    An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    fDIO-mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV1524

    Plasmid

    #111431

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

    Plasmid

    #75470

    Purpose

    Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Reversed ChR2(H134R)-mCherry
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn FLEx-FRT hM4Di-mCherry

    Plasmid

    #161576

    Purpose

    Expression of hM4Di-mCherry in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1393

    Plasmid

    #111430

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn FLEx-FRT hM3Dq-mCherry

    Plasmid

    #161575

    Purpose

    Expression of hM3Dq-mCherry in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-TO-DEST

    Plasmid

    #106348

    Purpose

    Destination vector for gateway cloning. Developed for Flp-In T-REx system.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Type

    Empty backbone

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid

    #49098

    Purpose

    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Tags

    GFP and acceptor peptide (AP)

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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