We narrowed to 3,455 results for: aaas

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LEP-shTAF2.1635

Plasmid

#105561

Purpose

retrovirally express TAF2 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF2 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHS1398

Plasmid

#205971

Purpose

MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-puro human lipin 1-1 shRNA

Plasmid

#32019

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDECKO_TFRC_B

Plasmid

#72626

Purpose

Expresses two gRNAs targeting the TFRC promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward TFRC

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-UAG site)

Plasmid

#170138

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(UAG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

Plasmid

#149588

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shFAK #1

Plasmid

#198757

Purpose

conditional knockdown of FAK

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

Insert

shFAK #1 (PTK2 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-YAP-WW1-RRK

Plasmid

#104306

Purpose

bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated

Depositor

Marius Sudol

Insert

YAP WW1 domain (YAP1 Human)

Tags

GST

Expression

Bacterial

Mutation

WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…

Promoter

tac

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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976 pSG5L HA PTEN 360-364A

Plasmid

#10751

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

360-364A (SSSTS to AAAAA)

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
PX458_HLF_1

Plasmid

#86301

Purpose

Encodes gRNA for 3' target of human HLF

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HLF (HLF Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13238

Plasmid

#219911

Purpose

The base plasmid of TUNEYALI for TF48

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF48 ( YALI1_C18396g) and homologous arm matching TF48

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13250

Plasmid

#219921

Purpose

The base plasmid of TUNEYALI for TF60

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF60 (YALI1_B16239g) and homologous arm matching TF60

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13249

Plasmid

#219920

Purpose

The base plasmid of TUNEYALI for TF59

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF59 ( YALI1_C25877g) and homologous arm matching TF59

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAflaB

Plasmid

#149589

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

Plasmid

#138150

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

Plasmid

#223175

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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gRNA-CasRx_SD1-pSV40-TagBFP

Plasmid

#221004

Purpose

Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

CasRx 30nt processed direct repeat with SD1 mutation

Use

CRISPR

Expression

Mammalian

Mutation

DR1 A>T

Promoter

U6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCFD3-ovoD1

Plasmid

#111142

Purpose

Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

Depositor

Norbert Perrimon

Article

Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

Insert

pCFD3-ovoD1 (ovo Fly)

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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