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We narrowed to 9,067 results for: sgRNA

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Showing: 981 - 1000 of 9067 results

  1. c3GIC9

    Plasmid
    #62191
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. Pyogenes Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62191-plasmid-map-sequence-id-156341

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  3. pMZ381

    Plasmid
    #59899
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469
    Depositor
    Mikel Zaratiegui
    Publication
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59899-plasmid-map-sequence-id-94872

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  5. eGFPbait-E2A-KalTA4-pA donor vector

    Plasmid
    #61069
    Purpose
    for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)
    Depositor
    Filippo Del Bene
    Publication
    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    eGFPbait
    KalTA4
    Use
    Zebrafish expression
    Tags
    E2A
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61069-plasmid-map-sequence-id-99003

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  11. pAL-rfp_T

    Plasmid
    #74075
    Purpose
    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR
    Depositor
    Timothy Lu
    Publication
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA rfp (T)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74075-plasmid-map-sequence-id-141966

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  12. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Yunde Zhao
    Publication
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    Yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    51056-plasmid-map-sequence-id-77326

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  15. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid
    #102851
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3X Flag and NLS
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102851-plasmid-map-sequence-id-198995

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  16. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid
    #133792
    Purpose
    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St3Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Muller et al. Molecular T…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133792-plasmid-map-sequence-id-268589

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  19. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133344
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA_cpaA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133344-plasmid-map-sequence-id-266084

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Showing: 981 - 1000 of 9067 results