We narrowed to 21,628 results for: ESC;

1. pAAV-hSyn-FRISZ-TM

   Plasmid

   #176886

   Purpose

   AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

   Depositor

   Huiwang Ai

   Article

   Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

   Insert

   Ig kappa-FRISZ-PDGFR TM
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ZJOM103

   Plasmid

   #133664

   Purpose

   Integration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-NtXNtS1R1AtR2NtB2

   Plasmid

   #160881

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcS-S1, rbcX, raf1, raf2, bsd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcS-S1: T7, chaperones: T7

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

   Plasmid

   #85070

   Purpose

   Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   PH-PLCD1 (PLCD1 Human)
   

   Use

   Tags

   IRES-sYFP2-PH and mScarlet-H

   Expression

   Mammalian

   Mutation

   PLCD aa 1–170

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH4687

   Plasmid

   #200783

   Purpose

   Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   ins-22 (ins-22 Nematode)
   

   Use

   Tags

   pHiuorin

   Expression

   Worm

   Mutation

   Promoter

   Pnpr-4

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. ZJOM96

   Plasmid

   #133663

   Purpose

   Integration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC26-mTagBFP2 (SEC26 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4136464

   Plasmid

   #149534

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. mEmerald-H2B-C-10

   Plasmid

   #54112

   Purpose

   Localization: Nucleus/Histones, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pKanCMV-CX4.3-7aa-mClover3

    Plasmid

    #74260

    Purpose

    Mammalian expression of C-terminal fusion of mClover3 to CX43

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Connexin 43
    

    Use

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. shNTC

    Plasmid

    #73549

    Purpose

    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shNTC
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pNIC28-BSA4-G-L11

    Plasmid

    #127830

    Purpose

    Production of recombinant human ribosomal protein L11

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPL11 (RPL11 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ALIX(P801G)-Strep

    Plasmid

    #180024

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX(1-868, P801G)-Strep (PDCD6IP Human)
    

    Use

    Tags

    Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. attB-­GFP-­Poly(A)

    Plasmid

    #65524

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZJOM30

    Plasmid

    #133673

    Purpose

    Integration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ERG6-GFP (ERG6 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRZ-mNanog-YNL

    Plasmid

    #65717

    Purpose

    mNanog reporter plasmid (expresses YNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Nanog promoter and YNL
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM135

    Plasmid

    #133659

    Purpose

    Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-17b_Unc104-KIF5B-HALO

    Plasmid

    #169996

    Purpose

    Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

    Depositor

    Ron Vale

    Article

    Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

    Insert

    Kinesin-like protein Unc-104 fused with KIF5B
    

    Use

    Tags

    HALO-tag, 6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CVS-N2c-tdTomato-ChIEF

    Plasmid

    #172370

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    tdTomato + ChIEF
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209660

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    GFAP

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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