We narrowed to 22,531 results for: scr

1. CROP_C9_Puro_DNMT3A

   Plasmid

   #183283

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   DNMT3A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB22

   Plasmid

   #122244

   Purpose

   Lentivirus delivery for stable expression of AsCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Expression

   Mutation

   Promoter

   EFs

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV PIG Myb

   Plasmid

   #66988

   Purpose

   Retroviral expression plasmid for mouse Myb expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Myb (Myb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ141A2.0

   Plasmid

   #99896

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP_C9_Puro_PARP1

   Plasmid

   #183312

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PARP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIV URA PCP-NLS-GFPEnvy

   Plasmid

   #189941

   Purpose

   Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

   Depositor

   Tineke Lenstra

   Article

   Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

   Insert

   PCP-NLS-GFPEnvy
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas12j2 KRAB

   Plasmid

   #188508

   Purpose

   Expresses FLAG tagged dCas12j2 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D394A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV Flag cycT2b (P#1718)

   Plasmid

   #14599

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

   Insert

   cycT2 (CCNT2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   A137T compared to NP_490595.1

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-ML1N*2

   Plasmid

   #67678

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2
   

   Use

   Gateway multisite 3' entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2xVlk1-3xFlag

    Plasmid

    #172773

    Purpose

    mRNA synthesis for injection into embryos

    Depositor

    Edward De Robertis

    Article

    Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

    Insert

    Vlk1 (pkdcc.1.S Frog)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV2-hNCOA6:V5

    Plasmid

    #63585

    Purpose

    A subunit of the Trithorax-related (Trr) methyltransferase complex.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. actc1b- mKate2-KDEL-pA

    Plasmid

    #109494

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b- mKate2-KDEL-pA
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH6HTC_IGF2BP1-Halo

    Plasmid

    #175300

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. S3_001_026_MV2

    Plasmid

    #134422

    Purpose

    pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUB-Cas9-@GL1

    Plasmid

    #86779

    Purpose

    Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    sgRNA against GLABROUS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6-26 promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-FOXR2

    Plasmid

    #153151

    Purpose

    Expression of Flag tagged human FOXR2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXR2 (FOXR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. actc1b-mKate2-rab27a

    Plasmid

    #109617

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab27a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab27a (rab27a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UNC50-myc

    Plasmid

    #104382

    Purpose

    Expresses UNC50-myc wild-type in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

    Insert

    UNC50 (UNC50 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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