We narrowed to 14,715 results for: flag

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P628_SpCas9-RecA

Plasmid

#87263

Purpose

Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

Depositor

Yonglun Luo

Article

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CBh

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS-LL/RK-NLS/D307A

Plasmid

#185459

Purpose

Inducible nuclear cGAS with D307A mutation

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

L157R, L159K, D307A

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTomo-AurA

Plasmid

#26293

Depositor

Inder Verma

Article

Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Insert

aurora kinase A (AURKA Human)

Use

Lentiviral

Tags

Flag

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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GreenT-EC - GPI

Plasmid

#193946

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Tags

GPI-anchor and Igk_secretion - HA-Flag

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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LNCX BMP-RII

Plasmid

#12641

Depositor

Rik Derynck

Article

Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

Insert

BMP-RII (BMPR2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only
MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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R4227X-F

Plasmid

#21371

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG (following D4226)

Expression

Mammalian

Mutation

Changed R4227 to stop codon

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
vas-Cas9.874Z

Plasmid

#112687

Purpose

Express hSpCas9 under vasa promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

vasa

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2430

Plasmid

#36829

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC2-TALEN_Left (Hdac2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEXP1/Joseph2-Multitagged Plasmid

Plasmid

#226724

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pEXP1/Joseph2

Tags

6xHis, HA, Myc, GFP, Flag, V5

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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P823_eSpCas9(1.1)-RecA

Plasmid

#87264

Purpose

Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

Depositor

Yonglun Luo

Article

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Tags

FLAG

Expression

Mammalian

Promoter

CBh

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
TAL2451

Plasmid

#36840

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HMGA2-TALEN_Right (Hmga2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEntr-Mef2aflbio

Plasmid

#32970

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Mef2a (MEF2A Human)

Use

Gateway

Tags

flag-bio

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
1198 pAdTrack FKHR

Plasmid

#9036

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR (FOXO1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pMSCV-WT-mAbl

Plasmid

#69027

Purpose

Retroviral vector encoding WT mouse c-Abl

Depositor

Jean Wang

Article

Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

Insert

mouse Abl-Type IV (wild type)

Use

Retroviral

Tags

FLAG tag inserted at Internal SalI site (2934 bp)

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
GAL4-BRD4

Plasmid

#21943

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag and GAL4-DNA-BD

Expression

Bacterial

Mutation

aa 1209-1362

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
SETMAR-Cas9

Plasmid

#186701

Purpose

Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETMAR-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pOZC-PALB2

Plasmid

#133552

Purpose

PALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.

Depositor

David Livingston

Article

Xia et al Mol Cell. 2006 Jun 23. 22(6):719-29.

Insert

PALB2 (PALB2 Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

Plasmid

#25846

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA-W37I

Tags

Flag and His

Expression

Mammalian

Mutation

W37I

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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