We narrowed to 27,542 results for: CAT

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FgH1tUTG_mup53_Ex4

Plasmid

#85534

Purpose

Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 N

Plasmid

#158370

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 N (N )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pQTEV-YWHAE

Plasmid

#31562

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pVenus-N-SLC35B2

Plasmid

#155038

Purpose

Transient expression of SLC35B2-Venus

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

Venus

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP1

Plasmid

#67862

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP1 (TRMT10C Human)

Tags

His

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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MBD_R44Q-IFP2.0C

Plasmid

#215749

Purpose

Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSO221 Phis-72 BirA GFP

Plasmid

#45100

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

BirA::GFP
unc-119 (unc-119 Nematode)

Tags

GFP

Expression

Worm

Promoter

his-72 and unc-119

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-Akt-STOPS

Plasmid

#175009

Purpose

Plasma membrane-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBS hIhh (CT#402)

Plasmid

#13995

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Ihh (IHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GFP

Plasmid

#41614

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-tdTomato

Plasmid

#106949

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2A-Akt-STOPS

Plasmid

#175010

Purpose

Akt-STOPS targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-mCherry-Akt-STOPS

Tags

Histone H2A and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-RNMT

Plasmid

#112709

Purpose

Expresses N-terminally GST-tagged human RNMT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

GST and thrombin cleavage site

Expression

Bacterial

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPm-DAD

Plasmid

#25410

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

mDia2 Amino Acids 1031-1171; Q1091P mutation

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-PAX9

Plasmid

#75083

Purpose

[Edit] retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

full length, wild type, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pEY51

Plasmid

#191054

Purpose

mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mec-3 (mec-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRKVSV Rac

Plasmid

#50535

Depositor

Kenneth Yamada

Article

Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

Insert

small GTPase (RAC1 Human)

Tags

2X VSV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
PE_CO-Mini-C

Plasmid

#190337

Purpose

Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini-C, PCSK9 +1A insertion pegRNA

Use

AAV

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET24a_SMOXdel

Plasmid

#186785

Purpose

C-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acids 503-512

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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