We narrowed to 59,498 results for: %s

1. pcDNA 3.1-nsp7

   Plasmid

   #201026

   Purpose

   Expresses SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-nsp7

   Plasmid

   #201025

   Purpose

   Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Promoter

   CMV-tet-ON

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDA2ScIfm

   Plasmid

   #193786

   Purpose

   Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Frame-shifted CI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLTATAA synthetic promoter carrying binding sites…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sha3Cas9

   Plasmid

   #192136

   Purpose

   Expresses Sha3Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

   Insert

   Sha3Cas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTA1cI-T3

   Plasmid

   #193789

   Purpose

   Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Truncated version of Lambda CI, named T3, where the first two codons are deleted
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLTetO-1

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

   Plasmid

   #192133

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-KY
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (K390A, Y446A)

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

   Plasmid

   #192132

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-Y
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (Y446A)

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWT35S-AtOEP7(1-50aa)-VC155

   Plasmid

   #194046

   Purpose

   Transient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
   

   Tags

   AtOEP7 1–50aa

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWT35S-AtOEP7(1-50aa)-VN154

   Plasmid

   #194045

   Purpose

   Transient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
   

   Tags

   AtOEP7 1–50aa

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ265K

    Plasmid

    #173997

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    R33A in rAPOBEC1 and D10A in zCas9

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYI006

    Plasmid

    #170840

    Purpose

    FRO6p::GUSPlus::tOCS in pFP100

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    FRO6p::GUSPlus::tOCS
    

    Expression

    Plant

    Promoter

    FRO6

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNB_EA_R1CiM_4EGFP_R3EGFPi2

    Plasmid

    #185968

    Purpose

    Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

    Depositor

    Sangram Bagh

    Article

    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

    Insert

    Molecular IMPLY gate
    

    Use

    Synthetic Biology

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Rock1-GBD (840-110 aa) in pEGFPN1

    Plasmid

    #187279

    Purpose

    Express EGFP-Rock1 GTPase-binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Rock1 (Rock1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    GBD (84-110aa)

    Promoter

    CMV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNB_EA_R3tdTomatoi2

    Plasmid

    #185969

    Purpose

    Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

    Depositor

    Sangram Bagh

    Article

    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

    Insert

    Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
    

    Use

    Synthetic Biology

    Promoter

    PR

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CBA-Con/Fon-EYFP-WPRE

    Plasmid

    #186752

    Purpose

    AAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    EYFP
    

    Use

    AAV

    Promoter

    CBA

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

    Plasmid

    #181861

    Purpose

    FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AKAR (mScarlet-I)
    

    Tags

    mScarlet-I and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWP001

    Plasmid

    #178596

    Purpose

    pAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiT

    Depositor

    Robert Fagan

    Article

    Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

    Insert

    CBD-SmBiT/LgBiT
    

    Expression

    Bacterial

    Promoter

    Pxyl

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-RiboJ-mTFP1-T7

    Plasmid

    #173225

    Purpose

    Encodes mTFP fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    mTFP1
    

    Use

    Cell-free system

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFGC-T7RibJ-RRvT

    Plasmid

    #173226

    Purpose

    Encodes RRvT fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    RRvT
    

    Use

    Cell-free system

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pJH3137

    Plasmid

    #179617

    Purpose

    Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    GCaMP6
    

    Tags

    RFP

    Expression

    Worm

    Promoter

    Punc-4

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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