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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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MG15-luc (mut p53 binding sites)

Plasmid

#16443

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pFEndoNA

Plasmid

#174724

Purpose

Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

Depositor

Jukka Finne

Article

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-E504K-T2A-EGFP

Plasmid

#197037

Purpose

Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2-E504K

Use

Lentiviral

Expression

Mammalian

Mutation

E504K

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-DsRed-Express2

Plasmid

#111251

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTD-NTwinStrep_Km

Plasmid

#45938

Purpose

use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

6xHis and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pJH4687

Plasmid

#200783

Purpose

Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pREDIA

Plasmid

#51625

Purpose

E. coli genome modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-mito-eqFP650

Plasmid

#174179

Purpose

Express eqFP650 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

eqFP650 with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(WT)

Plasmid

#182659

Purpose

Expresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

wild type mouse neurofilament light chain (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato

Plasmid

#244921

Purpose

iATPSnFR2.A95K variant with non-responsive tdTomato

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.tdTomato

Use

AAV

Mutation

A95K

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCarCBA

Plasmid

#117754

Purpose

Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCarCBADE

Plasmid

#117757

Purpose

Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised CarCBA, Protein CarD, Ferredoxin CarE

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

Plasmid

#117656

Purpose

Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Inserts

sfCherry2(1-10)
sfCherry2(11)-SpyCatcher002

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-EGFP

Plasmid

#65252

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-mito-roGFP

Plasmid

#174181

Purpose

Express roGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

roGFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pREDKI

Plasmid

#51626

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN-sDarken

Plasmid

#184796

Purpose

Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

sDarken

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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