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pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163044

Purpose

CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-H3.3-YFP

Plasmid

#8693

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3.3A (His3.3A Fly)

Tags

YFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

Plasmid

#211839

Purpose

Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Mutation

R181A R185A

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

Plasmid

#211841

Purpose

Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA and mCherry

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PfliC-GFPLVA

Plasmid

#23342

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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L1-P941L

Plasmid

#15498

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-P941L (L1CAM Human)

Expression

Bacterial

Mutation

Proline 941 changed to Leucine

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163062

Purpose

Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Gateway entry vector

Tags

mOrange2

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP FAT

Plasmid

#50517

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase, domain

Tags

GFP

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
KA2628_pGL4-UBCmin-luc2/Tbx3-B'

Plasmid

#124201

Purpose

Luciferase reporter driven by the Tbx3 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Tbx3 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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Rix-PGK-Tom-W

Plasmid

#25813

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

Rix-PGK-Tom-W

Use

Lentiviral

Mutation

tdTomato from Tsien lab

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Prx2(C172A)

Plasmid

#158228

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2(C172A)

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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L1-R184Q

Plasmid

#15501

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-R184Q (L1CAM Human)

Expression

Bacterial

Mutation

Arginine 184 changed to Glutamine

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFLIPglu-2uDelta13

Plasmid

#12990

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16F D152A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

D152A mutation in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pWZL-neo delta-p85

Plasmid

#10888

Depositor

William Hahn

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

delta-p85 (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Mutation

Deletion of aa 478-513

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK2

Plasmid

#38201

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
ptagBFP-LacI

Plasmid

#103839

Purpose

Flourescent marker for lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-STOP-IRES-Puro

Plasmid

#120558

Purpose

Expresses control HA-birA* in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

birA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

R118G

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mWnt1

Plasmid

#176486

Purpose

mouse Wnt1 cDNA in TetO backbone

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

mouse Wnt1 cDNA (Wnt1 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_TRAC

Plasmid

#164993

Purpose

Expression of gRNA targeting TCRalpha constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRAC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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px330 VRER

Plasmid

#101729

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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