176,531 results

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pYES2-Ub-M-GFP

Plasmid

#11952

Depositor

Nico Dantuma

Publication

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-M-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Methionine …

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FLAG.PKCzeta.T410A

Plasmid

#10801

Depositor

Alex Toker

Publication

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Activation loop inactive: T410A (Thr 410 to Ala)

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGEX-2T SHP1 WT

Plasmid

#8594

Depositor

Ben Neel

Publication

Plutzky et al Proc Natl Acad Sci U S A 1992 Feb 1;89(3

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Available since

June 21, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJ3 SHP2 WT

Plasmid

#8317

Depositor

Ben Neel

Publication

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBABE-puro SHP2 E76A

Plasmid

#8331

Depositor

Ben Neel

Publication

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E76A (activated mutant)

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p416 72Q GPD

Plasmid

#1179

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Publication

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Expression

Mammalian

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT3.0

Plasmid

#208717

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Has service

AAV8

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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ACBE-Q

Plasmid

#204608

Purpose

Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

Depositor

Dali Li

Publication

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Publication

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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V5-LOV-Turbo-NES

Plasmid

#199545

Purpose

expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Publication

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NES and V5

Expression

Mammalian

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-iCasp9

Plasmid

#199441

Purpose

Express inducible Caspase9 in mammalian cells

Depositor

Chao Tang

Publication

Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

Insert

inducible Caspase9 (CASP9 Human)

Use

Lentiviral

Tags

DmrB and mCherry

Expression

Bacterial and Mammalian

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pM-Cas9

Plasmid

#196325

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding SpCas9 in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Mutation

See depositor comments below

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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LM627

Plasmid

#191551

Purpose

LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

Depositor

David Baker

Publication

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

SNAC-tag, HIS-tag

Expression

Bacterial

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Publication

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJR101

Plasmid

#187241

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti EF1a GFP-mCherry-mPlin1

Plasmid

#189005

Purpose

Fluorescent reportor for lipophagy

Depositor

Atsushi Hoshino

Publication

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

GFP, mChery, Plin1 (Plin1 Mouse)

Use

Lentiviral

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HDRT_TRAC_CD19-CAR

Plasmid

#183473

Purpose

Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

Depositor

Dimitrios Wagner

Publication

Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

Inserts

TRAC (TRAC Human)
CD19-specific CAR transgene insert
TRAC (TRAC Human)

Use

Unspecified

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LentiGuide-puro-NLS-GFP

Plasmid

#185473

Purpose

Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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