Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 21,628 results for: ESC;

Showing: 10141 - 10160 of 21628 results

  4. ZJOM108

    Plasmid
    #133665
    Purpose
    Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133665-plasmid-map-sequence-id-292111

    Enlarge

  5. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

    Enlarge

  7. pET28a Gx-NL2

    Plasmid
    #169107
    Purpose
    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169107-plasmid-map-sequence-id-334716

    Enlarge

  9. pTEV5_ RpMatB_3

    Plasmid
    #78207
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A T167A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A T167A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    78207-plasmid-map-sequence-id-152439

    Enlarge

  16. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Article
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Use
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

    Enlarge

  17. pGBW-m4046931

    Plasmid
    #145706
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145706-plasmid-map-sequence-id-307566

    Enlarge

Showing: 10141 - 10160 of 21628 results