We narrowed to 59,498 results for: %s

1. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107321

   Purpose

   Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1333S) is fused to SaCas9

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21a - Ec-ClpX D144L

   Plasmid

   #111514

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Aspartatic acid 144 to Leucine

   Promoter

   T7

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET22b-ecDHFR-D27S/Y100F

   Plasmid

   #110827

   Purpose

   Bacterial expression of E.coli DHFR D27S/Y100F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   D27 mutated to S27, Y100 mutated to F100

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MDH1-PGK-FLAG-TAT-86-D

   Plasmid

   #35976

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "D" HIV clade

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MDH1-PGK-FLAG-TAT-86-C

   Plasmid

   #35982

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "C" HIV clade

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMflCT-o4

   Plasmid

   #101313

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   cat resistance gene
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMflT-o1

   Plasmid

   #101309

   Purpose

   Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Insert

   oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSN21

   Plasmid

   #100106

   Purpose

   expresses a chimeric OCT4-activator fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (POU5F1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSN61

   Plasmid

   #100122

   Purpose

   expresses putative OCT4 DBD with a T7 tag

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   vexGFP-P2A-putative OCT4 DBD (138-289) with a T7 tag (POU5F1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSN35

    Plasmid

    #100120

    Purpose

    expresses a chimeric OCT4-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSN31

    Plasmid

    #100116

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSN32

    Plasmid

    #100117

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR CMVie-Lifeact-mRuby2 L1-R5

    Plasmid

    #84389

    Purpose

    Multisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-Zeo-NKX2-8

    Plasmid

    #75090

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-8 (NKX2-8 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without native 3' UTR

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mKO-Alpha-Actinin-19

    Plasmid

    #57831

    Depositor

    Michael Davidson

    Article

    Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP eIF2beta C-term (aa 89-331)

    Plasmid

    #45901

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 aa89-331 (Eif2s2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 89-331

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pRSETC-PITPbeta (splice variant 1) K61A

    Plasmid

    #58271

    Purpose

    bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCI Flag Smad3 NL* EPSM

    Plasmid

    #27005

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-203; T179V

    Available Since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pCI Flag Smad3 NL* WT

    Plasmid

    #27042

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-203

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only