Skip to main content
Addgene
Showing: 1001 - 1020 of 4688 results

  2. pCAT024

    Plasmid
    #171636
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BFP
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171636-plasmid-map-sequence-id-337539

    Enlarge

  3. pFH6

    Plasmid
    #86555
    Purpose
    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    U6-26p::sgRNA scaffold
    Use
    CRISPR; Subcloning
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis U6-26 promoter
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86555-plasmid-map-sequence-id-176427

    Enlarge

  7. MS2-adRNA (5'UAG)

    Plasmid
    #170126
    Purpose
    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125
    Depositor
    Prashant Mali
    Article
    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:
    Insert
    MS2-ADAR2(5'UAG)-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and mouse U6
    Available since
    Jan. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    170126-plasmid-map-sequence-id-336437

    Enlarge

  9. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid
    #68432
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68432-plasmid-map-sequence-id-125560

    Enlarge

  11. pU6_T7_INT[6xminimal_Puf1]

    Plasmid
    #107608
    Purpose
    Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    6x minimal Puf1 motif
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107608-plasmid-map-sequence-id-207490

    Enlarge

  14. MS2-adRNA (3'UAG)

    Plasmid
    #170128
    Purpose
    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125
    Depositor
    Prashant Mali
    Article
    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:
    Insert
    MS2-ADAR2(3'UAG)-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and mouse U6
    Available since
    May 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    170128-plasmid-map-sequence-id-337251

    Enlarge

Showing: 1001 - 1020 of 4688 results