We narrowed to 44,084 results for: cha

1. pFA6a-TRP1-PGAL1-GFP

   Plasmid

   #41615

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP(S65T), TRP1 reverse orientation, and yeast AD…

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAV0756

   Plasmid

   #133508

   Purpose

   Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV0764

   Plasmid

   #133520

   Purpose

   Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV0773

   Plasmid

   #133519

   Purpose

   Expression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV0835

   Plasmid

   #133512

   Purpose

   Expression of mCherry tagged CHD domain to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV0816

   Plasmid

   #133510

   Purpose

   Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-mCherry-N1-slowChloC

   Plasmid

   #85462

   Purpose

   Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Peter Hegemann

   Article

   Wietek et al Science. 2014 Mar 27.

   Insert

   slowChloC
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E90R, T159C, D156N

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-TRP1-PGAL1-GST

   Plasmid

   #41612

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GST, TRP1 reverse orientation, and yeast ADH1 ter…

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH-HT_ygiF-FL

   Plasmid

   #127795

   Purpose

   Expresses full-length ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF (ygiF Escherichia coli)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VV228: sdChR(C138S E154A)-TS in fck

    Plasmid

    #58523

    Purpose

    Expresses sdChR(C138S E154A) in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S, E154A)-TS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine; changed Glutamate…

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. myc-SpEAF pBacPAK8

    Plasmid

    #15320

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    ELL associated factor (eaf1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pJET-TtrpC

    Plasmid

    #213467

    Purpose

    Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Terminator of tryptophan synthase of Aspergillus nidulans
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJET-PgpdA

    Plasmid

    #213466

    Purpose

    Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Promoter of the glyceraldehyde-3-phosphate dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJET-PtrpC

    Plasmid

    #213465

    Purpose

    Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Promoter of tryptophan synthase of Aspergillus nidulans
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KBB556

    Plasmid

    #185108

    Purpose

    1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160A

    Depositor

    Ken Belanger

    Article

    Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

    Insert

    POM152
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    1st 200 amino acids (N-term + transmembrane domai…

    Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KBB553

    Plasmid

    #185107

    Purpose

    1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182L

    Depositor

    Ken Belanger

    Article

    Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

    Insert

    POM152
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    1st 200 amino acids (N-term + transmembrane domai…

    Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV0747

    Plasmid

    #133481

    Purpose

    Expression in fission yeast from promoter ppom1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV0748

    Plasmid

    #133482

    Purpose

    Expression in fission yeast from promoter ppak1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV0605

    Plasmid

    #133503

    Purpose

    Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV0746

    Plasmid

    #133480

    Purpose

    Expression in fission yeast from promoter prga3 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-SpEAF pBacPAK8

    Plasmid

    #15319

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    ELL associated factor (eaf1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

23. KA1042_pPB-hCMV*1-Esrrb-IV

    Plasmid

    #124177

    Purpose

    piggyBac-based Dox-inducible expression of Esrrb

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb-IRES-Venus (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KA2880_pPB-TREtight-FLAG-Esrrb-InO

    Plasmid

    #124178

    Purpose

    piggyBac-based Dox-inducible expression of FLAG-Esrrb

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    FLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KA2944_pPB-TREtight-Esrrb-FLAG-InO

    Plasmid

    #124179

    Purpose

    piggyBac-based Dox-inducible expression of Esrrb-FLAG

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-His3MX6-PGAL1

    Plasmid

    #41607

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. VPR-ps-dSpCas9

    Plasmid

    #102855

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    dSpCas9, pdDronpa1.2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS and VP64-p64-Rta transcription activator

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIRES:hTRAAK

    Plasmid

    #133080

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full length

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pZac2.1 gfaABC1D-LifeAct-GFP

    Plasmid

    #190199

    Purpose

    Expresses the small actin binding peptide, LifeAct, with a GFP reporter

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    LifeAct-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. OSCA2.3

    Plasmid

    #136595

    Purpose

    Expresses the mechanosensitive channel OSCA2.3 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA2.3 (HYP1 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFA6a-kanMX6-PGAL1-3HA

    Plasmid

    #41608

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFA6a-kanMX6-PGAL1

    Plasmid

    #41605

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

    Plasmid

    #182824

    Purpose

    Cre-dependent expression of cytosolic APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    APEX.NES-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFA6a-kanMX6-PGAL1-GFP

    Plasmid

    #41614

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-kanMX6-PGAL1-GST

    Plasmid

    #41611

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HM3C NaVAe1_p pET24b

    Plasmid

    #127854

    Purpose

    Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1p
    

    Use

    Tags

    3C Cleavage site, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJT11

    Plasmid

    #98308

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3ClLIS1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFA6a-His3MX6-PGAL1-3HA

    Plasmid

    #41610

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. HpMscS

    Plasmid

    #78310

    Purpose

    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

    Depositor

    Douglas Rees

    Article

    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

    Insert

    Mechanosensitive channel of small conductance
    

    Use

    Tags

    6x His Tag and FLAG Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pIRTetR-GAL80

    Plasmid

    #185853

    Purpose

    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJT10R

    Plasmid

    #98307

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 N127W

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. pJT2

    Plasmid

    #98306

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 N127W

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. HM3C NaVSp1_p pET24b

    Plasmid

    #127852

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    3C Cleavage site, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

    Plasmid

    #52878

    Purpose

    Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    CaMKIIa

    Available Since

    July 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. myc-SpELL pBacPAK8

    Plasmid

    #15318

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

48. FLAG-SpEAF 60-251 pBacPAK8

    Plasmid

    #15321

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    EAF1 (eaf1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    N terminal deletion lacking first 59 amino acids …

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

49. p11_AaC60αβ/C20

    Plasmid

    #229506

    Purpose

    Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Chaperonin 60α
    Chaperonin 60β
    Chaperonin 20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. MCUb-mVenus-pLJM

    Plasmid

    #236789

    Purpose

    MCUB tagged with mVenus for FRET / MW change for stoichiometry

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB (MCUB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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