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pcDNA3-KLF3

Plasmid

#49100

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

Insert

Krüppel-Like Factor 3(KLF3)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNAIIIB HA JNK

Plasmid

#61630

Purpose

mammalian expression of JNK

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

JNK1 beta1 isoform (MAPK8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-EphB4-Fc

Plasmid

#200986

Purpose

Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB4

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 NHE-RF

Plasmid

#11635

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

NHE-RF (NHERF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)_CMVtrunc_877

Plasmid

#105828

Purpose

A modified pcDNA3.1- vector with reduced expression activity of a truncated CMV promoter

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+):RGS4

Plasmid

#31157

Depositor

Henry Lester

Article

Doupnik et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

Regulator of G Protein Signaling 4 (Rgs4 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-ΔN-RNMT

Plasmid

#112708

Purpose

Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG and HIV Rev nuclear export signal (NES)

Expression

Mammalian

Mutation

deleted amino acids 2-120, mutated active-site D2…

Promoter

CMV

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Lyn-OeNL_pcDNA3

Plasmid

#89528

Purpose

Orange color variant of bright luminescent protein, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Lyn

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-deltaCKAR

Plasmid

#31526

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-lynD3cpV

Plasmid

#37472

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Use

Tags

the myristoylation and palmitoyla- tion sequence …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 V5 mDispatched WT 2x HA

Plasmid

#126410

Purpose

Expression of V5 Dispatched WT HA in mammalian cell lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

m-Dispatched1 (Disp1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

V5 iserted between A106 and A107

Promoter

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BREP

Plasmid

#172337

Purpose

BRET-Based Bioluminescent Red Protein

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TN-XXL pcDNA3

Plasmid

#45797

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Use

Tags

ECFP and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5kin

Plasmid

#65245

Purpose

Expression of HA tagged ERK5 kinase domain in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5kin (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 410-817

Promoter

CMV

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 DLC1

Plasmid

#24266

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1, DLC1 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-hAIM2-T7

Plasmid

#51538

Purpose

Express T7-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10A-V5

Plasmid

#35939

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt8A

Plasmid

#35916

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8A (Wnt8a Human)

Use

Tags

Expression

Mammalian

Mutation

Silent Mutation A (600) --> G

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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