We narrowed to 1,648 results for: sfGFP

1. pY71-tet.I.6

   Plasmid

   #133528

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pY71-tet.I.5

   Plasmid

   #133527

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY71-tet.I.3

   Plasmid

   #133525

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pY71-tet.I.2

   Plasmid

   #133524

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY71-tet.I.4

   Plasmid

   #133526

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY71-tet.I.1

   Plasmid

   #133523

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJBL7049

   Plasmid

   #167219

   Purpose

   cell-free arsenic sensing reporter

   Depositor

   Julius Lucks

   Insert

   pArs-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJBL7050

   Plasmid

   #167220

   Purpose

   cell-free mercury sensing reporter

   Depositor

   Julius Lucks

   Insert

   pMer-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJBL7051

   Plasmid

   #167221

   Purpose

   cell-free cadmium sensing reporter

   Depositor

   Julius Lucks

   Insert

   pCad-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJBL7053

    Plasmid

    #167222

    Purpose

    cell-free lead sensing reporter

    Depositor

    Julius Lucks

    Insert

    pPbr-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJBL7054

    Plasmid

    #167223

    Purpose

    cell-free copper sensing reporter

    Depositor

    Julius Lucks

    Insert

    pCue-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ-Cas9-Act3.0

    Plasmid

    #178954

    Purpose

    It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ-dzCas9-Act3.0

    Plasmid

    #158414

    Purpose

    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAP05

    Plasmid

    #186261

    Purpose

    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

    Plasmid

    #158585

    Purpose

    Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    membrane localization signal-tagBFP-wrmScarlet11
    

    Use

    Tags

    tagBFP

    Expression

    Yeast

    Mutation

    GGGS linker

    Promoter

    TEF

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ-dSpRY-Act3.0

    Plasmid

    #158416

    Purpose

    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid

    #154141

    Purpose

    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    R887E

    Promoter

    SFFV

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ-dpcoCas9-Act3.0

    Plasmid

    #158408

    Purpose

    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ-SpRY-Act3.0

    Plasmid

    #178957

    Purpose

    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge