175,508 results

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pET15bHis6Tnp

Plasmid

#79807

Purpose

Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

Depositor

Keji Zhao

Article

Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

Insert

His6Tnp

Tags

His

Expression

Bacterial

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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px459 VQR

Plasmid

#101715

Purpose

sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGF1-4eCOL2A1

Plasmid

#97210

Purpose

Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

Depositor

Ryan Porter

Article

De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

Insert

4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

COL2A1 regulatory elements

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ220 (AsCpf1)

Plasmid

#86208

Purpose

AsCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

AsCpf1

Use

Gateway compatible cpf1 vectors

Tags

5' and 3' NLS

Expression

Plant

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ233 (dLbCpf1-SRDX)

Plasmid

#86211

Purpose

Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR

Tags

5' and 3' NLS and SRDX repressor

Expression

Plant

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-RRvT

Plasmid

#87364

Purpose

Expresses RRvT, a tandem heterodimer red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

RRvT

Tags

His-Tag

Expression

Bacterial

Promoter

pBad (Arabinose)

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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AAV9:cTNT::3Flag-hYAP S127A

Plasmid

#86558

Purpose

To produce cardiac specific adeno-associated virus expressing activated human YAP.

Depositor

William Pu

Article

Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

Insert

YAP (YAP1 Human)

Use

AAV

Tags

3Flag

Mutation

Serine 127 was mutated into Alanine, to activate …

Promoter

cTNT

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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CMV Marina-T2A-nls-mCherry

Plasmid

#74216

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

fluorescent protein voltage sensor

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Expression

Bacterial

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mRuby2 BlastR

Plasmid

#84384

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK CA Rac1

Plasmid

#84605

Purpose

Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pGWB414

Plasmid

#74808

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pscAAV-CAG-RLuc

Plasmid

#83280

Purpose

recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

Insert

Renilla Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagGFP2

Plasmid

#67166

Purpose

CRISPaint universal donor for TagGFP2 fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagGFP2

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLV_Zika_Flag_NS4A

Plasmid

#79636

Purpose

Third generation Self-inactivating lentivector expressing NS4A from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS4A

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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35S-sfGFP-nosT

Plasmid

#80129

Purpose

Transient expression vector for sfGFP in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Type

Empty backbone

Tags

sfGFP

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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