165,688 results

1. PEA1-Puro

   Plasmid

   #171991

   Purpose

   Delivers all prime editing nickase components in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPM47 (UPR-RFP CEN/ARS URA3)

   Plasmid

   #20132

   Depositor

   Feroz Papa

   Article

   Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

   Insert

   UPR-RFP
   

   Expression

   Yeast

   Available Since

   Feb. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Neo-Bam APC

   Plasmid

   #16507

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-HA-ER Y537S

   Plasmid

   #49499

   Purpose

   Expresses HA-tagged ER Y537S mutant in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tyrosine 537 to Serine

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

   Plasmid

   #223149

   Purpose

   Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-mTurquoise2-Caspase-1-mNeonGreen

   Plasmid

   #166784

   Purpose

   FRET analysis (pro caspase-1 cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Green Pegassos

   Plasmid

   #163114

   Purpose

   Expresses biosensors for pyruvate in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

   Insert

   Green Pegassos
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGIG-6xHIS.Mm.RPE65-eGFP

   Plasmid

   #41019

   Depositor

   John Nickerson

   Insert

   RPE65 (Rpe65 Mouse)
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-MATR3

   Plasmid

   #32880

   Depositor

   Yossi Shiloh

   Article

   Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

   Insert

   MATR3 Matrin3 (MATR3 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRES-hSphK2-EGFP

    Plasmid

    #84371

    Purpose

    Expression of exogenous human SphK2 in mammalian cells

    Depositor

    Anthony Don, Hugh Rosen

    Article

    Don et al J Biol Chem. 2007 May 25;282(21):15833-4

    Insert

    SPHK2 (SPHK2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

11. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh; U6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. dg265_pENTR_CoChRCel

    Plasmid

    #66103

    Purpose

    Entry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    CoChR
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 SIRT7_H187Y

    Plasmid

    #53151

    Purpose

    expression vector for catalytically inactive human Sirtuin7

    Depositor

    Katrin Chua

    Article

    Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

    Insert

    Sirt7 (SIRT7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H187Y, catalytic inactive

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

    Plasmid

    #191172

    Purpose

    Human ADAR1, p150 isoform in plant-expressable format.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)
    

    Tags

    3FLAG and HIS

    Expression

    Plant

    Mutation

    WT

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDestTol2CG2--3.5ubb:plin2-tdtomato

    Plasmid

    #172534

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2 mCherry-Atl1

    Plasmid

    #86678

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO ChETA-EYFP

    Plasmid

    #26968

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T, H134R

    Promoter

    EF1alpha

    Available Since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJFRC7-20XUAS-IVS-mCD8::GFP

    Plasmid

    #26220

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET3a-OSF-CypA

    Plasmid

    #79039

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCF525-EF1a-Hygro-P2A-mCherry-lenti

    Plasmid

    #115796

    Purpose

    Expresses mCherry in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1a promoter

    Available Since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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