We narrowed to 14,379 results for: TIM

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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PP363

Plasmid

#78984

Purpose

255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

rHGH
IFNa2b

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF-CcAvs4

Plasmid

#188808

Purpose

Codon optimized CcAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEBB FLAG XIAP D148A/W310A

Plasmid

#25685

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

FLAG

Expression

Mammalian

Mutation

D148A W310A

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pEBB HA XIAP delta BIR

Plasmid

#25686

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

delta BIR Contains only aa 331-497

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pEBB XIAP Delta RING

Plasmid

#40828

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

Delta RING: contains aa 1-448

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
FH95pUGW (B3)

Plasmid

#74012

Purpose

lentiviral expression of EGFP and Psd95 shRNA

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1-puro/hpb1(short)

Plasmid

#59973

Purpose

integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

Depositor

Timothy Springer

Insert

human integrin b1 (short) (ITGB1 Human)

Tags

Base coiled-coil and His-tag

Expression

Mammalian

Promoter

EF1a promoter

Available Since

Sept. 24, 2014

Availability

Academic Institutions and Nonprofits only
pEBB XIAP H467A

Plasmid

#25680

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

H467A

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pVP65KR-gb-SacB(-)

Plasmid

#171646

Purpose

Equipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.

Depositor

Young Kee Chae

Article

Chae et al Protein Pept Lett. 2021;28(12):1398-1407

Insert

MBP-mCherry-8xHis-SacB(-)

Tags

8xHis

Mutation

internal HindIII site in the SacB gene was remove…

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK054

Plasmid

#171326

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

QS (QA-1S) codon optimized, quinic acid repressor

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEBB XIAP D148A/W310A

Plasmid

#25678

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

D148A W310A

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pJAID6A

Plasmid

#165046

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pfMDH_H303A

Plasmid

#67730

Purpose

Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed histidine 303 to alanine

Promoter

T7

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_D230A

Plasmid

#67725

Purpose

Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed aspartate 230 to alanine

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_N191A

Plasmid

#67724

Purpose

Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed asparagine 191 to alanine

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_K295A

Plasmid

#67726

Purpose

Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed lysine 295 to alanine

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
FH95pUP95PDZGW (C6)

Plasmid

#74022

Purpose

lentiviral expression of Psd95 shRNA and Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

PDZ

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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