We narrowed to 22,530 results for: scr

1. pDEST-actc1b-ccdb-mKate2

   Plasmid

   #109731

   Purpose

   Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Type

   Empty backbone

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST-actc1b-mKate2-ccdb

   Plasmid

   #109732

   Purpose

   Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Type

   Empty backbone

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5F Smad1

   Plasmid

   #12622

   Depositor

   Rik Derynck

   Article

   Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

   Insert

   Smad1 (SMAD1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pPT-PCR C2

   Plasmid

   #141247

   Purpose

   PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Algal Research, 2020. 50, 102014

   Insert

   Mitochondrial Genome of Phaeodactylum tricornutum
   

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Repeat region removed, additional minor mutations…

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-HsSENP7

   Plasmid

   #71470

   Purpose

   Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 7 (SENP7 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pColE1_sgRNA_M7NS_AmpR

   Plasmid

   #119157

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pColE1_sgRNA_M2S_AmpR

   Plasmid

   #119154

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ptcALPS gag-gfp Blasti

   Plasmid

   #115806

   Purpose

   Express gag-gfp fusion driven by TK promoter

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TK

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1-2x

   Plasmid

   #172282

   Purpose

   Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence

   Depositor

   Antony K Chen

   Article

   Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

   Insert

   EGFP-N1-2x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1-4x

    Plasmid

    #172283

    Purpose

    Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-4x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dCas12j3 KRAB

    Plasmid

    #188507

    Purpose

    Expresses FLAG tagged dCas12j3 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. actb2-lifeact-mKate2

    Plasmid

    #109750

    Purpose

    Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2 fused to the actin binding lifeact sequence
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFB-Flag-MyoD

    Plasmid

    #25994

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJZC593

    Plasmid

    #62319

    Purpose

    sgRNA with MS2-PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + MS2-PP7 RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLib6.6B

    Plasmid

    #176666

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-flag-UTR

    Plasmid

    #183234

    Purpose

    Mammalian expression empty backbone

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3E-rab32a

    Plasmid

    #109689

    Purpose

    Multisite gateway vector for 3' tagging with rab32a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab32a (rab32a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flag-FOXD2

    Plasmid

    #153117

    Purpose

    Expression of Flag tagged human FOXD2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXD2 (FOXD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-G6-mCherry

    Plasmid

    #66533

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter G6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - G6

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge