161,273 results

1. pDest472 SHP-1 WT

   Plasmid

   #21804

   Depositor

   Stephen Shaw

   Article

   Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

   Insert

   SHP-1 (PTPN6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

2. qTAG-C-mStayGold-Puro-PXN

   Plasmid

   #227317

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-Ii_VSVG-SBP-mCherry

   Plasmid

   #65301

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. β3-integrin-YFP

   Plasmid

   #26653

   Depositor

   Jonathan Jones

   Article

   Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

   Insert

   β3-integrin (ITGB3 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0091

   Plasmid

   #142457

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nsp12-EGFP

   Plasmid

   #165116

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp12 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAST-pHuji-GINKO2

   Plasmid

   #177117

   Purpose

   Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

   Depositor

   Robert Campbell

   Article

   Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

   Insert

   pHuji-GINKO2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70 promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNS35 (multiAsCas12a piggyBac)

   Plasmid

   #217336

   Purpose

   piggyBac expression of multiAsCas12a

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   CAG

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. HLA_A2402

    Plasmid

    #165010

    Purpose

    Expression of HLA-A*24:02 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV 4xSTAR-mTagBFP2-blast

    Plasmid

    #125239

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTagBFP2 (codon optimized)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

    Plasmid

    #107387

    Purpose

    Lentiviral expression of IKZF1-V1-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1-V1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-V5-SBP-C1-HshnRNPM

    Plasmid

    #64924

    Purpose

    Expresses V5- and SBP-tagged hnRNPM in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    hnRNPM (HNRNPM Human)
    

    Use

    Tags

    V5 and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pESF140_HisSpy_DDB1∆B

    Plasmid

    #124213

    Purpose

    Insect cell expression vector for His6, Spy tagged hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Use

    Tags

    His6-Spy

    Expression

    Insect

    Mutation

    internal deletion of BPB domain to aid crystalliz…

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5-Smad7-HA

    Plasmid

    #11733

    Depositor

    Jeff Wrana

    Article

    Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

    Insert

    SMAD7 (SMAD7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. mKO2-SLBP(18-126)

    Plasmid

    #83914

    Purpose

    Fluorescent probe for S/G2 cell cycle transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mKO2-SLBP(18-126) (SLBP Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pREDawn-AmpR-MCS

    Plasmid

    #188978

    Purpose

    For red light-induced bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge