177,604 results
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Plasmid#199545Purposeexpresses LOV-Turbo in the mammalian cytosol, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNES and V5ExpressionMammalianPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmScarlet3-alphaTubulin_C1
Plasmid#189768PurposeConstruct for labelling microtubules in mammalian cells with mScarlet3-alphatubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet3-alphaTubulin
ExpressionMammalianPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert6xCRE-CMVmin-luc2
ExpressionMammalianPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
Tags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR101
Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti EF1a GFP-mCherry-mPlin1
Plasmid#189005PurposeFluorescent reportor for lipophagyDepositorInsertGFP, mChery, Plin1 (Plin1 Mouse)
UseLentiviralAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-puro-NLS-GFP
Plasmid#185473PurposeMammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
AKT-translocation-mStrawberry reporter
Plasmid#158685PurposeThis plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRatiometric pHLuorin2 fused to GPI anchor
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)
Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcytoEKAR4
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLIN2-V5-APEX2
Plasmid#170573PurposeA C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2DepositorAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-28c S2
Plasmid#171920PurposeExpress RNA-based (p)ppGpp sensorDepositorInsertRNA-based (p)ppGpp sensor
ExpressionBacterialPromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mScarlet
Plasmid#169410PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarletDepositorInsertspLeft
mScarlet proper
UseUnspecifiedAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FLAG-SFPQ
Plasmid#166960PurposeExpresses FLAG-tagged SFPQ protein in pcDNA3.1DepositorAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorPublicationInsertLgBiT-PhoCl1-SmBiT-MBP
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-mTert
Plasmid#162555PurposeOverexpression of TertDepositorAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTet-O-ASCL1-T2A-PuroR
Plasmid#162345PurposeLentiviral expression of ASCL1 under the control of the TetON promoterDepositorAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by