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pCDNA5/FRT_CoA-Snifit(V97T)

Plasmid

#197947

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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LR (bact: HyPer)

Plasmid

#162069

Purpose

Used for the diffuse visualization of H2O2 production in cells.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

beta actin 2 (actb2 Zebrafish)

Use

Gateway Cloning

Tags

HyPer

Promoter

T3

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW tdTomato-T2A-mTurquoise2

Plasmid

#118439

Purpose

Produces equimolar expression of tdTomato and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tdTomato-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal Katushka

Plasmid

#104029

Purpose

Plasmid encoding 2ndVal Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka

Use

Low copy

Expression

Bacterial

Mutation

Katushka with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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p414TEF_Hsp82_CycTER

Plasmid

#61711

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(92Q)-S776D

Plasmid

#21757

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 92 Q(Glutamines) and…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pDEST27-GST-ATXN1 FL(30Q)-S776D

Plasmid

#21755

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pZSm47a luxR RBS

Plasmid

#48891

Purpose

ArsR-regulated expression of luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

luxR

Use

Synthetic Biology

Expression

Bacterial

Promoter

pArsR

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GST

Plasmid

#39286

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-GST

Plasmid

#39287

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
TALE12

Plasmid

#35427

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE12

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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TALE11

Plasmid

#35426

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE11

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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TALE10

Plasmid

#35425

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE10

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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TALE6

Plasmid

#35421

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE6

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTH371-SUP45-N58A

Plasmid

#29378

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

N58A mutant gene including genomic sequence 1432 …

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH397-SUP45-I222S

Plasmid

#29388

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

I222S mutant gene (corresponding to the sup45-2 a…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ro2

Plasmid

#24503

Depositor

Bruce Conklin

Article

Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

Insert

RASSL Ro2 (OPRK1 Human)

Tags

FLAG and NLS from prolactin

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Available Since

June 16, 2010

Availability

Academic Institutions and Nonprofits only
pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

Plasmid

#216244

Purpose

Express AtAFB2.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

AtAFB2.F74A-mCherry

Use

CRISPR, TALEN, and Unspecified ; Human safe harbo…

Tags

mCherry

Expression

Mammalian

Mutation

F74A mutation

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-mNeonGreen-CAMSAP3

Plasmid

#191322

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

mNeonGreen-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE50

Plasmid

#90389

Purpose

PPARg/RARa - PPARg gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ACO1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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