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pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211863

Purpose

Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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IL1_1

Plasmid

#207623

Purpose

Low copy plasmid expressing mTurquoise2 under the TDH3p promoter

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Insert

mTurquoise2

Expression

Yeast

Promoter

ScTDH3

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC8-NLS

Plasmid

#207395

Purpose

Expresses AcrIC8 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC8-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIE2-NLS

Plasmid

#207396

Purpose

Expresses AcrIE2 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIE2-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSECB sgCASP9

Plasmid

#211525

Purpose

Deletes CASP9

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgFADD

Plasmid

#211527

Purpose

Deletes FADD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCKL1

Plasmid

#211523

Purpose

Deletes UCKL1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCKL1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCK2

Plasmid

#211522

Purpose

Deletes UCK2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUGP2

Plasmid

#211521

Purpose

Deletes UGP2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUGP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

Plasmid

#209090

Purpose

mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:γB2-EC5-VenusΔN3C9-P3

Plasmid

#209092

Purpose

mVenus-inserted PcdhgB2

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:γB2-EC1-G4S-mTQ2ΔC6

Plasmid

#209091

Purpose

mTurquoise2-inserted PcdhgB2

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pZa pLtetO CsgA-SpyTag

Plasmid

#204503

Purpose

Bacterial expression of CsgA with C-terminal SpyTag peptide and GS linker

Depositor

Urartu Seker

Article

Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

Insert

CsgA

Use

Synthetic Biology

Tags

6xHis, SpyTag

Expression

Bacterial

Promoter

pLtetO

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ016_MTB_CoChR_KGC_eGFP_ER2

Plasmid

#209277

Purpose

Ubiquitously expresses CoChR in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

CoChR_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ035_MTB_gtACR2_KGC_eGFP_ER2

Plasmid

#209278

Purpose

Ubiquitously expresses gtACR2 in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

gtACR2_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ037_MTB_FR-GECO1c

Plasmid

#209279

Purpose

Ubiquitously expresses FR-GECO1c in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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FB406

Plasmid

#203627

Purpose

Promoter of the TAKA-amylase A gene from A. oryzae, maltose/starch-inducible.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PamyB

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB407

Plasmid

#203628

Purpose

Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Pef1A

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB408

Plasmid

#203629

Purpose

Promoter of the ubiquitin ligase gene from P. digitatum (PDIG_07760).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

P07760

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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