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Agrp-pGL3

Plasmid

#17552

Depositor

Domenico Accili

Article

Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Insert

Agrp (Agrp Mouse)

Use

Luciferase

Mutation

Contains ~400bp of the mouse Pomc gene promoter, …

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Stat5

Plasmid

#139832

Purpose

doxycycline-inducible expression of mouse Stat5 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Stat5 (Stat5a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX APLP1

Plasmid

#30141

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Like Protein 1 (APLP1 Human)

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pGL4.10-Claudin-1

Plasmid

#46387

Purpose

Claudin-1 promoter reporter assay

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

CLDN1 Promoter (CLDN1 Human)

Use

Luciferase

Promoter

CLDN1 promoter

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-nv5-ER beta

Plasmid

#22770

Depositor

Donald McDonnell

Article

Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

Insert

estrogen receptor beta (ESR2 Human)

Tags

v5

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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FUW-H2B-mTurquoise

Plasmid

#193093

Purpose

constitutive expression of H2B-mTurquoise in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

H2B-mTurquoise

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-AviTag-DogCatcher-MBP

Plasmid

#171928

Purpose

Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogCatcher-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

DogCatcher added to N-terminus of MBP via a GSGES…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXL2

Plasmid

#153135

Purpose

Expression of Flag tagged human FOXL2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXL2 (FOXL2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pgWIZ-SGCAgl

Plasmid

#131456

Purpose

Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

SGCA (SGCA Human)

Tags

3xFLAG

Expression

Bacterial and Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2-356bp

Plasmid

#16292

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -344 to +12 relative to first transcript…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-NGLY1-Puro

Plasmid

#181916

Purpose

Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

N-glycanase 1 (NGLY1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-FOXC1

Plasmid

#153114

Purpose

Expression of Flag tagged human FOXC1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC1 (FOXC1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PssaG-GFPLVA

Plasmid

#23343

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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S1m tagged DANCR

Plasmid

#138585

Purpose

For in vivo tagging

Depositor

Yin-Yuan Mo

Article

Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

Insert

DANCR (DANCR Human)

Use

Lentiviral

Tags

S1m tag

Promoter

MSCV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTetOne-DsRed-cNLS

Plasmid

#138528

Purpose

Inducible expression of DsRed-Express2 with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

DsRed-Express2

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE3Gs

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Cyclin D1

Plasmid

#19927

Depositor

Yue Xiong

Article

Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

Insert

Cyclin D1 (CCND1 Human)

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-NMD3

Plasmid

#221296

Purpose

Expression of NMD3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

NMD3 (pLxIS) (Nmd3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PIPK1 gamma 90

Plasmid

#22299

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pCW-CCNE1-HA-puro

Plasmid

#212669

Purpose

Express tagged cyclin E1

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

CCNE1 (CCNE1 Human)

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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