162,682 results

1. RFP-(SUMO)3

   Plasmid

   #127150

   Purpose

   Mammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30b-7+-SrtA

   Plasmid

   #105602

   Purpose

   expressed 7+ sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7+ mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGK-T7/2-CD44v2-10 (fl)

   Plasmid

   #137824

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v2-10 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length and mutations A282T, T483A, N535D, S5…

   Promoter

   PGK

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRT801

   Plasmid

   #68112

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Clover

   Plasmid

   #40259

   Purpose

   GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Insert

   Clover GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP(C3)-Nup153

   Plasmid

   #64268

   Purpose

   GFP-tagged mammalian expression of Nup153

   Depositor

   Birthe Fahrenkrog

   Article

   Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

   Insert

   NuP153 (NUP153 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-Ii_SBP-mCherry-Golgin84

   Plasmid

   #65304

   Purpose

   synchronize trafficking of Golgin-84 from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-mCherry-Nanobody (LaM-2)

   Plasmid

   #162276

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

   Insert

   mCherry-Nanobody (LaM-2)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF_dCas9

    Plasmid

    #68416

    Purpose

    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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