We narrowed to 13,974 results for: CRISPR-Cas9

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pMYT095

Plasmid

#198866

Purpose

CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT096

Plasmid

#198867

Purpose

Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRVV651

Plasmid

#87631

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV647

Plasmid

#87630

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV653

Plasmid

#89474

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV598

Plasmid

#87629

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCRRNAcos

Plasmid

#78492

Purpose

Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

Depositor

David Bikard

Article

Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

Insert

tracrRNA

Expression

Bacterial

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSH624-ssr

Plasmid

#199241

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

single-stranded DNA recombineering

Expression

Bacterial

Promoter

lacIq-Ptrc

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSH124-ssr

Plasmid

#198025

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Ap/Cb resistance cassette

Expression

Bacterial

Promoter

ApR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pACDura3

Plasmid

#138711

Purpose

Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Insert

ura3 gene (URA3 Budding Yeast)

Expression

Bacterial

Mutation

optimized ura3 gene for expression in Escherichia…

Promoter

pEM7 promoter

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-NLS-tdMCP-mCherry

Plasmid

#99909

Purpose

For the expression of NLS-tdMCP-mCherry

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-tdMCP-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Asc

Plasmid

#74285

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG AsiSI/MluI

Plasmid

#74286

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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