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pCMV- κLight1.3

Plasmid

#201223

Purpose

Express kLight1.3 biosensor in mammalian cells

Depositor

Lin Tian

Article

Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

Insert

κLight1.3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDGM3B

Plasmid

#110809

Purpose

contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

Depositor

David Russell

Article

Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

Insert

AAV3B cap genes, AAV2 rep genes, adenovirus helper genes

Use

AAV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Blast-H2BC11

Plasmid

#207762

Purpose

Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-BRAF

Plasmid

#116719

Purpose

Lentiviral expression of BRAF

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowR

Plasmid

#132668

Purpose

Expresses ShadowR in mammalian cells

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2019 Aug 19;9(1):12072. doi: 10

Insert

ShadowR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Tags

DsbC-6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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AY27_pU6.gRNA.CLYBL

Plasmid

#199238

Purpose

Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. pyogenes gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human

Plasmid

#238130

Purpose

This plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

TMEM106B (TMEM106B Human)

Use

Lentiviral

Tags

Blasticidin-P2A-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK 3XFlag_4D5-5_CAR-mCherry

Plasmid

#247508

Purpose

Expresses a CAR against HER2 and mCherry

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

CAR against Her2 and mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pOpen-Tthlig

Plasmid

#165569

Purpose

Taq DNA Ligase

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Tth DNA Ligase

Use

Synthetic Biology

Available Since

Oct. 25, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCXLE-mp53DD

Plasmid

#41859

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp 53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET28c-F30-Broccoli

Plasmid

#66788

Purpose

Expresses Broccoli in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Broccoli in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pJL-TRBO

Plasmid

#80082

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-BRT17

Plasmid

#45358

Depositor

Scott Banta

Article

Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

Insert

17 Repeat Beta Roll Tag

Tags

Maltose Binding Protein (MBP)

Expression

Bacterial

Promoter

Ptac

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Vimentin-7

Plasmid

#56439

Purpose

Localization: Intermediate Filaments, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

Vimentin (VIM Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2 Notch1 Full Length-6MT

Plasmid

#41728

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Full Length (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

L1997V; Contains murine Notch-1 protein up to ami…

Available Since

Dec. 18, 2012

Availability

Academic Institutions and Nonprofits only

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untargeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45215

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Aurora B FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Promoter

t7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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