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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218867

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179074

Purpose

Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TEF2p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-VAPA(1-242) K87D/M89D

Plasmid

#18875

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GFP

Expression

Mammalian

Mutation

double point mutant K87D / M89D

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pR2Q358y

Plasmid

#101028

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE Doc2A-GFP KI

Plasmid

#131478

Purpose

Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Doc2a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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NGL-1-myc/pEGFP-N1

Plasmid

#197314

Purpose

Plasmid expressing an antigen targeting Neuroligin-1

Depositor

Eunjoon Kim

Insert

Neuroligin-1 (Nlgn1 Rat)

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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mPlum-Lysosomes-20

Plasmid

#55982

Purpose

Localization: Lysosome Membrane, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

LAMP1 (Lamp1 Rat)

Tags

mPlum

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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p75 NTR

Plasmid

#24091

Depositor

Moses Chao

Insert

p75NTR (Ngfr Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pBJ1-rat Beta2m

Plasmid

#121670

Purpose

Expresses rat β-2-microglobulin in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat beta-2- microglobulin (B2m Rat)

Expression

Mammalian

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-oA ONE-GO

Plasmid

#204330

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-oA ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-Sec22b-shR

Plasmid

#208358

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations introduced to render shRNA …

Promoter

CMV

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TO-PV-NLS-Dsred

Plasmid

#16341

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-DsRed-NLS (Pvalb Rat)

Tags

Myc and NLS

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-evoA1-seBE4max-IRES

Plasmid

#174699

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

evoA1-seBE4max-IRES

Tags

myc tag

Expression

Mammalian

Mutation

UGI-E11K

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-K52R

Plasmid

#40813

Depositor

Natalie Ahn

Article

Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

Insert

MAPK1 (Mapk1 Rat)

Tags

His6

Expression

Mammalian

Mutation

K52R

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26567

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons a10, 31a, 37b, and 46. Deletion…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pET14b PHAS-I

Plasmid

#15679

Depositor

John Lawrence

Article

Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

Insert

PHAS-I (Eif4ebp1 Rat)

Tags

6xHis

Expression

Bacterial

Mutation

Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 βarr1 S412D Flag

Plasmid

#42196

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412D (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412D

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only
pSIN ReP5-GFP-GluR1

Plasmid

#24004

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR1 (Gria1 Rat)

Use

Sindbis virus production

Tags

GFP

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
GFP-ERK2 K52R

Plasmid

#118298

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 K52R (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

K52R

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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