2,226 results

1. pcDNA3.1(+):RGS4

   Plasmid

   #31157

   Depositor

   Henry Lester

   Article

   Doupnik et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

   Insert

   Regulator of G Protein Signaling 4 (Rgs4 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

2. LUC2 (L/Sec/deltaP)

   Plasmid

   #112145

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and d…

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.1(-) rat C/EBP beta (LAP)

   Plasmid

   #12558

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta (Cebpb Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

4. Igfbp1-promoter/pGL3

   Plasmid

   #12146

   Depositor

   Domenico Accili

   Article

   Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

   Insert

   IGFBP1 promoter (Igfbp1 Rat)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains the canonical insulin response sequence …

   Promoter

   Available since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pPA016 (delta 86-130 Parkin)

   Plasmid

   #102357

   Purpose

   expresses Rat Parkin with residues 86-130 deleted

   Depositor

   Kalle Gehring

   Article

   Sauve et al EMBO J. 2015 Oct 14;34(20):2492-505. doi

   Insert

   Parkin (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deleted residues 86 to 130

   Promoter

   tac

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-GINA3

   Plasmid

   #74979

   Purpose

   Expresses GST tagged GINA3 protein in bacteria.

   Depositor

   Pradipta Ghosh

   Article

   Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

   Insert

   Rattus norvegicus G protein subunit alpha i3 (Gnai3) (Gnai3 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEAK-cKal7

   Plasmid

   #145801

   Purpose

   expresses Kalirin-7 with the c-front in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

   Insert

   cKalirin-7 (Kalrn Rat)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   EIF2a

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEAK-bKal7

   Plasmid

   #145800

   Purpose

   expresses Kalirin-7 with the b-front in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

   Insert

   bKalirin-7 (Kalrn Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EIF2a

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-GFP-PQRv3-RFP

   Plasmid

   #73951

   Purpose

   Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

   Depositor

   Brian Chen

   Article

   Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

   Inserts

   sfGFP (superfolder GFP)
   RFP
   

   Use

   Tags

   A residual PQR peptide wii be left at the C-termi…

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin and Chicken beta actin (shared…

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

10. Geph CR shRNA

    Plasmid

    #121068

    Purpose

    shRNA targeting the coding region of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p70 S6 kinase

    Plasmid

    #1892

    Depositor

    Joseph Avruch

    Article

    Weng et al Mol Cell Biol 1995 May;15(5):2333-40.

    Insert

    p70 alpha1 S6 kinase (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pTrcHisA-rat-PTP1B

    Plasmid

    #35987

    Depositor

    Anna Huttenlocher

    Article

    Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

    Insert

    protein tyrosine phosphatase 1B (Ptpn1 Rat)
    

    Use

    Tags

    His and Xpress Epitope Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSIL-eGFP-shACTN1

    Plasmid

    #52676

    Purpose

    shRNA against α-actinin-1 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN1 (Actn1 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1/HisC 14-3-3 epsilon wt

    Plasmid

    #11942

    Depositor

    Michael Yaffe

    Article

    Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

    Insert

    Ywhae (Ywhae Rat)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pRS426-GFP-PLCdelta-PH

    Plasmid

    #71375

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    PLCdelta-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain

    Promoter

    Available since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. Rat SERCA1a (pMT2)

    Plasmid

    #75182

    Purpose

    Mammalian expression of ATP2A1

    Depositor

    Jonathan Lytton

    Article

    Wu et al Am J Physiol. 1993 Feb;264(2 Pt 1):C333-

    Insert

    ATP2A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSuperGFP-cdc42-prenyl siRNA

    Plasmid

    #22715

    Depositor

    Alaa El-Husseini

    Insert

    cdc42 siRNA (Rac1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DHFR-GFP-th-GluA1

    Plasmid

    #173008

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-GFP-th-GluA1
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge