We narrowed to 5,876 results for: nls

1. SARS-CoV-2_N_deltaCIDR

   Plasmid

   #157872

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDR

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaCIDR

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDIV489

   Plasmid

   #177697

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNS3

   Plasmid

   #131770

   Purpose

   PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   E490G to make Cry2 into Cry2Olig

   Promoter

   PADH1

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNS1

   Plasmid

   #131769

   Purpose

   PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   FUSN_Fusion Red_Cry2WT
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PADH1

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAT15485_Hypa-ABE8e

   Plasmid

   #174123

   Purpose

   Expresses ABE8e fused with Hypa-SpCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   Hypa-ABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDIV490

   Plasmid

   #177698

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Beta lactamase S68TAG

   Plasmid

   #68305

   Purpose

   Beta lactamase S68TAG gene

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   S68TAG beta lactamase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T7T3-PacI-Neto1

   Plasmid

   #87672

   Purpose

   In situ probe for mouse Neto1

   Depositor

   Oscar Marin

   Article

   Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

   Insert

   Neto1 (Neto1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   partial sequence, (1244 to 2278bp of NM_144946.4)

   Promoter

   T7/T3

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRO204

   Plasmid

   #233843

   Purpose

   MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-ABI-tENO1

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pTDH3-NLS-VP48-ABI-tENO1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRO279

    Plasmid

    #233845

    Purpose

    MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-1xOaf1-ABI-tENO1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-NLS-VP48-1xOaf1ABI-tENO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRO280

    Plasmid

    #233846

    Purpose

    MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-NLS-VP48-2xOaf1-ABI-tENO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAL008

    Plasmid

    #233849

    Purpose

    MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWN021

    Plasmid

    #233851

    Purpose

    MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWN059

    Plasmid

    #233853

    Purpose

    MoClo-YTK; ConL1/R2 cassette; Auxin systems; pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWN061

    Plasmid

    #233854

    Purpose

    MoClo-YTK; ConL1/R2 cassette; NS3 systems; pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Efg1-ΔDBD-Y115S/Y132S/Y139S

    Plasmid

    #216408

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y115S/Y132S/Y139S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 115, 132 and 139 to Serine, and …

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Efg1-ΔDBD-A51P/A56P/Q443P

    Plasmid

    #216402

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P/Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51, 56 and Glutamine 443 to Proli…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

    Plasmid

    #216403

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51, 56 and Glutamine 70, 443 to P…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Efg1-ΔDBD-Y4S/Y8S/Y16S

    Plasmid

    #216405

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y4S/Y8S/Y16S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 4, 8 and 16 to Serine, and delet…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Efg1-ΔDBD-Y64S/Y66S/Y69S

    Plasmid

    #216406

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y64S/Y66S/Y69S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 64, 66 and 69 to Serine, and del…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Efg1-ΔDBD-Y101S/Y104S/Y106S

    Plasmid

    #216407

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y101S/Y104S/Y106S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 101, 104 and 106 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Efg1-ΔDBD-Y401S/Y404S/Y409S

    Plasmid

    #216409

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y401S/Y404S/Y409S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 401, 404 and 409 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Efg1-ΔDBD-Y450S/Y452S/Y456S

    Plasmid

    #216410

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y450S/Y452S/Y456S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 450, 452 and 456 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Efg1-ΔDBD-Y478S/Y482S/Y484S

    Plasmid

    #216411

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y478S/Y482S/Y484S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 478, 482 and 484 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. JAB3479

    Plasmid

    #190417

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JAB3278

    Plasmid

    #190402

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. JAB3616

    Plasmid

    #190426

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JAB2601

    Plasmid

    #190388

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JAB2600

    Plasmid

    #190387

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a/hU6

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. JAB2599

    Plasmid

    #190386

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDIV492

    Plasmid

    #177700

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDIV491

    Plasmid

    #177699

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDIV488

    Plasmid

    #177696

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAT15484_HF-ABE8e

    Plasmid

    #174122

    Purpose

    Expresses ABE8e fused with SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HRP-1LC

    Plasmid

    #139116

    Purpose

    express the LC of HRP-1

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    181-346

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJ4M_HRP-1_FL

    Plasmid

    #139117

    Purpose

    express HRP-1 with a C-terminal maltose binding protein tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BII-Sh-PnGW-CMV

    Plasmid

    #133366

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BII-Sh-PnGW-CMV-Luc

    Plasmid

    #133367

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. BII-Sh-PnGW-Luc

    Plasmid

    #133360

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. kank1a_pJet1.2

    Plasmid

    #121978

    Purpose

    for generating kank1a riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank1a (si:dkey-146l2.1 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCR1323

    Plasmid

    #111125

    Purpose

    Expresses non-targeting_01224 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    Non-targeting gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28a-clbH-C-A2-PCP

    Plasmid

    #114157

    Purpose

    Expresses ClbH-C-A2-PCP

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Only contains the C-A2-PCP domains of ClbH

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3-myc-mCE(211-597)

    Plasmid

    #82462

    Purpose

    Expression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mCE(211-597) (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. FNpCDNA3

    Plasmid

    #45346

    Depositor

    Robert Oshima

    Type

    Empty backbone

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET28a-EGFP-CNA35

    Plasmid

    #61603

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with EGFP

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    EGFP and CNA35
    

    Use

    Tags

    His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMIA22

    Plasmid

    #214448

    Purpose

    Expresses BxbI-BP-NLS

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    BxbI
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCMV-Mm.cargo(Cas9)

    Plasmid

    #174865

    Purpose

    Expresses mouse Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by MmPeg10 UTRs
    

    Use

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge