We narrowed to 7,541 results for: URE

Showing: 1021 - 1040 of 7541 results

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Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFH3.2_mTagBFP2-MS2x12

Plasmid

#205558

Purpose

Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mTagBFP2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.MTRIA-OT

Plasmid

#184594

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Promoter

Human synapsin promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-YFP-DD

Plasmid

#96891

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-YFP-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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HAI

Plasmid

#71316

Purpose

Inducible expression of human arginase I in E. coli

Depositor

David Christianson

Article

DiCostanzo et al Proc Natl Acad Sci U S A. 2005 Sep 13;10

Insert

human arginase I (ARG1 )

Expression

Bacterial

Promoter

tac

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_dCas9-5xGCN4_Hygro

Plasmid

#235572

Purpose

Dox-inducible expression of dCas9-GCN4 & genomic integration

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

dCas9-5xGCN4

Use

CRISPR

Mutation

D10A; H840A

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPM48

Plasmid

#22854

Depositor

Feroz Papa

Article

Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Insert

eroGFP & UPR-RFP

Expression

Yeast

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-UtrCH

Plasmid

#98879

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

UtrCH

Tags

mNeonGreen

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-mRuby2 eFRET

Plasmid

#59174

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Article

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-mRuby2 eFRET

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pIgnaviCas9

Plasmid

#127595

Purpose

Expresses IgnaviCas9 in E. coli

Depositor

Stephen Quake

Article

Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

Insert

IgnaviCas9

Tags

6xHis Tag and Maltose binding protein

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd4

Plasmid

#42256

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 4 (Fzd4 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
AAV-Stuffer

Plasmid

#106248

Purpose

Destination vector for U6::gRNA expression cassette

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Type

Empty backbone

Use

AAV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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BglB in pET29b(+)

Plasmid

#55773

Purpose

Expresses beta-glucosidase

Depositor

Justin Siegel

Article

Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

Insert

Beta-glucosidase B (bglB Synthetic)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt7b

Plasmid

#42283

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt7b (Wnt7b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pCDB364

Plasmid

#110275

Purpose

N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Type

Empty backbone

Tags

10-His, TEV cleavage peptide, and mature DsbC (i.…

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a Halotag-CNA35

Plasmid

#131128

Purpose

Halotag-CNA35 peptide for Collagen labelling

Depositor

Samantha Stehbens

Article

Ju et al Nat Cell Biol. 2024 Sep;26(9):1520-1534.

Insert

Halotag-CNA35

Tags

Halotag

Expression

Bacterial

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPS1622

Plasmid

#8854

Depositor

Pamela Silver

Article

Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

Insert

Sec63-sGFP (SEC63 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

sGFP=S65T V163A mutant of GFP

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only