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Showing: 1021 - 1040 of 16706 results

  4. pTcI38-PRM-wasabi

    Plasmid
    #195554
    Purpose
    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38
    Depositor
    Mikhail Shapiro
    Article
    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2
    Inserts
    mWasabi
    TcI38
    Use
    Tags
    6x His and AANDENYADAS degradation tag
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    195554-plasmid-map-sequence-id-426585

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  6. Palpha2

    Plasmid
    #204441
    Purpose
    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.
    Depositor
    Keir Neuman
    Article
    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586
    Insert
    hOpa1 isoform 1 (OPA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    W771A K772A K773A R774A W775A L776A Y777A W778A K…
    Promoter
    CMV
    Available since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    204441-plasmid-map-sequence-id-404953

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  8. pET28a(+)-HisTim12_CtoA

    Plasmid
    #170281
    Purpose
    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2
    Insert
    yeast Tim12 (TIM12 Budding Yeast)
    Use
    Tags
    TEV-cleavable N-terminal His tag (on backbone)
    Expression
    Bacterial
    Mutation
    mutated two non-conserved Cys to Ala
    Promoter
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170281-plasmid-map-sequence-id-334955

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  20. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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Showing: 1021 - 1040 of 16706 results