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pSG5-FLAG-CaMKKbeta rat D311A

Plasmid

#32451

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D311A (S3V, G96R, and P108L also present)

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

Plasmid

#232879

Purpose

Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

5-Hydroxytryptamine Receptor 6 (HTR6 Human)

Use

Lentiviral

Tags

RGECO1.1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GCaMP6f-WPRE

Plasmid

#216275

Purpose

AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Expression

Mutation

Promoter

human Synapsin 1 (hSyn)

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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TAL3497

Plasmid

#47216

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-right (nox5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3496

Plasmid

#47215

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-left (nox5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat S572A

Plasmid

#33319

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S571A

Promoter

SV40

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha

Plasmid

#33330

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

CaMKKbeta N-terminus (1-164) was fused to body fo…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
ER-GCaMP6-150

Plasmid

#86918

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-150

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-210

Plasmid

#86919

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-210

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6f.WPRE.SV40

Plasmid

#100837

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Orai1-jGCaMP8f

Plasmid

#231630

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f (ORAI1 Human, Synthetic)

Use

Tags

jGCaMP8f

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6f.WPRE.SV40

Plasmid

#100836

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV2, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CAG

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6s.WPRE.SV40

Plasmid

#100843

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pcDNA6)

Plasmid

#26578

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Use

Tags

His6 and V5

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-HaloCaMP1a-EGFP

Plasmid

#138327

Purpose

chemigenetic calcium indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Has Service

AAV1 and AAV9

Insert

HaloCaMP1a

Use

AAV

Tags

EGFP, His-tag, and Nuclear Exclusion Signal

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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CanlonicSF

Plasmid

#178342

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

CanlonicSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGPTVII-Bar-U-MatryoshCaMP6s

Plasmid

#100024

Purpose

Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Use

Tags

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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a1Ic-HE3-pcDNA3

Plasmid

#45810

Depositor

Edward Perez-Reyes

Article

Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Insert

CACNA1I (CACNA1I Human)

Use

Making crna for oocyte injections

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.GCaMP6s.WPRE.SV40

Plasmid

#100844

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Mito-CanlonicSF/pCMV-myc-mito

Plasmid

#224465

Purpose

To explore mitochondrial lactate dynamics

Depositor

Alejandro San Martín

Article

Rauseo et al bioRxiv 2024.07.19.604361

Insert

CanlonicSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge