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pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-CDDR

Plasmid

#149355

Purpose

CRISPR/Cas9-based dual-fluorescent DSB repair reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

CDDR Reporter
Blasticidin resistance

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a and hPGK

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGB411

Plasmid

#134760

Purpose

pCambia CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pG3H-U6EC1

Plasmid

#134758

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA-U6, zCas9-EC1

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-PC

Plasmid

#92125

Purpose

Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9

Use

CRISPR; Trichoderma reesei expression

Promoter

Ppdc

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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HiFi Cas9

Plasmid

#188490

Purpose

Expresses FLAG tagged HiFi Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R691A

Promoter

EF1a

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDB4280

Plasmid

#98699

Purpose

A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMB63

Plasmid

#47945

Purpose

Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_MFE1

Plasmid

#84612

Purpose

Introduces DSB at MFE1 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pTU422

Plasmid

#178982

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Inserts

gRAMP
CRISPR array

Tags

Strep-Tag II SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB4906

Plasmid

#106150

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB6364

Plasmid

#106151

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMonAID_dCas9-PmCDA_Hyg_ALS

Plasmid

#91692

Purpose

Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A and H840A for dead Cas9

Promoter

2x35S

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-1

Plasmid

#161818

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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SpRY Cas9

Plasmid

#188491

Purpose

Expresses FLAG tagged SpRY Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFUGW Cas9-BFP

Plasmid

#127396

Purpose

Cas9 expression

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Insert

Cas9

Use

Lentiviral

Tags

BFP

Promoter

SVVF

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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