Addgene

We narrowed to 8,312 results for: sgrna

Showing: 1021 - 1040 of 8312 results

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_1

Plasmid

#190703

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_3

Plasmid

#190705

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 3

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_1

Plasmid

#190700

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_2

Plasmid

#190701

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_3

Plasmid

#190702

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 3

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.3 (H3f3b) sgRNA

Plasmid

#186931

Purpose

H3f3b sgRNA used for genomic targeting at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.1 (Hist1h3g) sgRNA

Plasmid

#186930

Purpose

Hist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hist1h3g sgRNA (H3c8 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Mcm2-2A mutation sgRNA

Plasmid

#186936

Purpose

sgRNA used for genetic mutation at Y81 and Y90 of Mcm2

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Mcm2-2A mutation sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

P(Z), synthetic promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAmreB

Plasmid

#149595

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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