-
-
VPS13C^halo∆1235-1748
Plasmid#187297PurposeExpress VPS13C truncation mutant in mammalian cellsDepositorInsertVPS13C truncation mutant (VPS13C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAmCyan1-C1-ADCY6
Plasmid#113905PurposeN-terminal Cyan Fluorescent tag in Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 (ADCY6 Human)
UseTagsCyanExpressionBacterial and MammalianMutationPromoterpCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a-QL
Plasmid#86076PurposeA mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.DepositorInserthRab8A (RAB8A Human)
UseTagsGFPExpressionMammalianMutationChanged glutamine 67 to leucinePromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh Halo Tag (JDW 1223)
Plasmid#224498PurposeGateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)DepositorInsertActin-Vhh-Halo-Tag
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTY-EF1a-wild type mKdm2b-Flag
Plasmid#61739PurposeExpress wild-type mouse histone demethylase Kdm2bDepositorInsertKdm2b (Kdm2b Mouse)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smV5-4x6T-WPRE
Plasmid#196416PurposeAstrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a-TN
Plasmid#86077PurposeA mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.DepositorInserthRab8A (RAB8A Human)
UseTagsGFPExpressionMammalianMutationChanged threonine 22 to asparginePromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-actin prom-dsRed-scramble shRNA
Plasmid#67879PurposeAAV vector to express dsRed sh RNA (scramble)DepositorInsertactin prom-dsRed-scramble shRNA
UseAAV and RNAiTagsExpressionMammalianMutationPromoterchicken beta actinAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-GBF1(E794K)
Plasmid#206416PurposeMammalian expression of hGBF1(E794K)DepositorInsertGBF1 (GBF1 Human)
UseTagsHAExpressionMammalianMutationE794KPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1 D471-472K-pHcRed C1
Plasmid#62961Purposefull length human Mucolipin-1 D471/472K cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRedExpressionMammalianMutationD471/472K (and two silent mutations T77 ACA inst…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
CD8-GFP-FKBP
Plasmid#100751PurposeExpression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.DepositorInsertCD8A (CD8A Human)
UseTagsFKBP and GFPExpressionMammalianMutationTruncatedPromoterCMVAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
aav-tnt-actn2-gfp
Plasmid#165034PurposeAAV-based delivery of ACTN2-GFP into cardiomyocytes in vivoDepositorInsertACTN2 (Actn2 Mouse)
UseAAVTagsGFPExpressionMutationPromotercTNTAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
hMLKL-Venus-Plasma
Plasmid#106081Purposeexpression in mammalian cellsDepositorInserthMLKL-Venus-Plasma (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsplasma membrane-targetting domain of H-Ras (aa 1-…ExpressionMammalianMutationPromoterdox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-NMHC-IIAdeltaIQ2
Plasmid#35690DepositorInsertMHC IIA (MYH9 Human)
UseTagsEGFP and GFPGenotRWExpressionMammalianMutationThe coding segment for aa 804-834 (corresponding …PromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-FNTA-FLAG
Plasmid#86858PurposeTo express FNTA with a FLAG tag at C terminus.DepositorInsertFNTA (FNTA Human)
UseTagsFLAGExpressionMammalianMutationThe sequence of the insert is isoform 2, which di…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsEDC4
Plasmid#66597PurposeExpresses lambdaN-HA-tagged EDC4 in mammalian cellsDepositorInsertEDC4 (EDC4 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-lck-smV5
Plasmid#196423PurposeCre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificityDepositorInsertLck-smV5
UseAAV and Cre/LoxTagsLckExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only
