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pBAD/HisB-Medium-FT

Plasmid

#31917

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Tags

His

Expression

Bacterial

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

araB

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Publication

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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p5069 pcDNA4c hBrd4 (471-730)

Plasmid

#14448

Depositor

Peter Howley

Publication

You et al Cell. 2004 Apr 30. 117(3):349-60.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Brd4 (471-730) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 471-730

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pJH200

Plasmid

#13327

Depositor

Martin Gellert

Publication

Lieber et al Genes Dev. 1987 Oct . 1(8):751-61.

Insert

recombination substrate

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
CRISPR-StAR4GN (Lenti)

Plasmid

#222693

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Publication

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-Caspase-1-mNeonGreen

Plasmid

#166784

Purpose

FRET analysis (pro caspase-1 cleavage) in living cells

Depositor

Hao Wu

Publication

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Publication

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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BK alpha subunit

Plasmid

#113566

Purpose

mouse BK channel alpha subunit expression vector

Depositor

Robert Brenner

Publication

Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kcnma1 (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSEMS-Halo7Tag-hFis

Plasmid

#111136

Purpose

hFis with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Publication

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human fission factor1 (FIS1 Human)

Tags

Halo7-tag

Expression

Mammalian

Mutation

*

Promoter

CMV

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-MyosinVa-Ctail

Plasmid

#110169

Purpose

Dominant negative effect by competing with MyosinVa C-terminal tail interactions

Depositor

Jose Esteban

Publication

Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Myosin Va (Myo5a Mouse)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

amino acids 1444-1853

Promoter

CMV

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CD8-mCherry-FRB

Plasmid

#100738

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Publication

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD8A (CD8A Human)

Tags

FRB and mCherry

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-TK1

Plasmid

#100544

Purpose

mammalian expression of thymidine kinase 1

Depositor

Yuh-Shan Jou

Publication

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

thymidine kinase 1 (TK1 Human)

Expression

Mammalian

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Publication

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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JWW-2 human chimeric monoclonal antibody

Plasmid

#66749

Purpose

Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

Depositor

Richard Roden

Publication

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-2 Heavy Chain
JWW-2 Light Chain

Expression

Mammalian

Promoter

mEF1 and rEF1

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Publication

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

Plasmid

#40343

Depositor

Alexander Dent

Publication

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RSV promoter

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

RSV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-Ha-Brca1 S1387A

Plasmid

#32532

Depositor

Michael Kastan

Publication

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Brca1 (BRCA1 Human)

Tags

HA

Expression

Mammalian

Mutation

S1387A

Promoter

CMV/Rabbit B-Globin

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pSVL(D2m)

Plasmid

#29336

Depositor

John Taylor

Publication

Kuo et al J Virol. 1989 May . 63(5):1945-50.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dimer of HDV genome with 2 nucleotide deletion in delta antigen

Expression

Mammalian

Mutation

Contains a dimer of the complete HDV genome excep…

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Publication

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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