We narrowed to 21,628 results for: ESC;

1. pMamCol1

   Plasmid

   #215184

   Purpose

   Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

   Depositor

   Krishna Kishore Inampudi

   Insert

   tsPurple
   

   Use

   AAV, CRISPR, and Synthetic Biology ; Recombinant …

   Tags

   SNAC-StrepII tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetON 3G Bidirectional for Mammalian and Trc with…

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-mTagBFP2-FRB-Rab5

   Plasmid

   #214272

   Purpose

   Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   mTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC

   Plasmid

   #213398

   Purpose

   transcriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine

   Depositor

   Katrin Willig

   Article

   Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

   Insert

   FingR for endogenous PSD95, transcriptinally regulated
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRMTK-clo1

   Plasmid

   #203919

   Purpose

   Cloning plasmid containing the BHR1 origin insert with a chloramphenicol resistance marker

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   BHR1 Origin of Replication
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   N/A

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAB-HDAC5(mut-H-Y)

   Plasmid

   #114398

   Purpose

   For PYL-HDAC5 H1006Y expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5 H1006Y
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   additional D593E compared to NCBI ref NP_005465.2

   Promoter

   CMV

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-dMASS_1_WPRE

   Plasmid

   #209765

   Purpose

   AAV virus production for neuronal expression of dMASS_1 under hSyn promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   dMASS_1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB(EXPR-PYL-DNMT1-NEO)

   Plasmid

   #114396

   Purpose

   For PYL-DNMT1 expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-DNMT1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209656

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95K

   Promoter

   CAG

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-HisD-BR1

   Plasmid

   #192816

   Purpose

   Expresses BR1 in bacteria

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   BR1 (BRI1 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYG205

    Plasmid

    #200836

    Purpose

    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives

    Depositor

    Boris Görke

    Article

    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

    Insert

    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P-BAD

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-THTAP-SPARTIN, residues 1-101

    Plasmid

    #180626

    Purpose

    Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPARTIN
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-101

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-THTAP-CALPAIN-7 1-165

    Plasmid

    #180610

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-65

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CALPAIN-7 residues 1-165

    Plasmid

    #180611

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-REPORT(PGK/CMV)-GRE

    Plasmid

    #172336

    Purpose

    Gal4 Response element

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-P19-EGFP

    Plasmid

    #196609

    Purpose

    siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + EGFP inverted repeat sequence
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-P19-NP

    Plasmid

    #196608

    Purpose

    siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + Influenza virus NP inverted repeat
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK BirA* 3xFLAG-NUP37 zeo

    Plasmid

    #192333

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-NUP37

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BirA*-3xFLAG-NUP37 (NUP37 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TT BirA*-3xFLAG-NUP37

    Plasmid

    #192311

    Purpose

    Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-NUP37

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BirA*-3xFLAG-NUP37 (NUP37 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NeuA.pJL1

    Plasmid

    #199115

    Purpose

    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

    Depositor

    Michael Jewett

    Article

    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

    Insert

    N-acylneuraminate cytodiylyltransfease (NeuA)
    

    Use

    Tags

    Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTC3cIfm

    Plasmid

    #193778

    Purpose

    Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Frame-shifted CI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO-1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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