We narrowed to 12,272 results for: cel.2;

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pYLL204

Plasmid

#53455

Purpose

Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Article

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)

Tags

HA

Expression

Mammalian

Mutation

Y814F

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pBluescript-mouse eIF2C2

Plasmid

#50354

Purpose

Mouse eIF2C2 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C2 (Ago2 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_CD19.4

Plasmid

#140101

Purpose

CRISPR-Cas9 Knock down

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

Plasmid

#176400

Purpose

Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

FRB-RGG-GFP-RGG-RGG

Tags

FRB and GFP

Expression

Yeast

Promoter

GAL1

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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-227 hTF (mutant NF-kB)

Plasmid

#15443

Depositor

Nigel Mackman

Article

Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

NF-kappaB binding domain mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
200 bp Axin2 basic

Plasmid

#25703

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
tetO-SMARCD3

Plasmid

#170701

Purpose

doxycycline-inducible overexpression of SMARCD3

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SMARCD3 (SMARCD3 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-SNAI2

Plasmid

#170702

Purpose

doxycycline-inducible overexpression of SNAI2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SNAI2 (SNAI2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1-pCR8-dCas9VP48

Plasmid

#48214

Purpose

dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP48 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP48

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-48xHSPA1B array

Plasmid

#236378

Purpose

Expression of array of 48 crRNAs targeting HSPA1B

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xHSPA1B crRNA array (HSPA1B Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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AA366

Plasmid

#215970

Purpose

Fragmid fragment: (Pol 2 promoter) Gal4-induced expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 800

Plasmid

#131147

Purpose

To investigate activity of a 800 bp region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Tags

luciferase

Expression

Mammalian

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 2.6

Plasmid

#131151

Purpose

To investigate activity of a 2.6 kb region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Tags

luciferase

Expression

Mammalian

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 500

Plasmid

#131150

Purpose

To investigate activity of a 500 bp region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-HA-TSAd-Y89XF

Plasmid

#84559

Purpose

mammalian expression of TSAd-Y89XF

Depositor

Anne Spurkland

Article

Granum et al J Biol Chem. 2008 Aug 8;283(32):21909-19

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Mutation

Y279F, Y290F, Y305F

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pSG5 N-HA SADBs shRNA resistant

Plasmid

#67157

Purpose

N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

Promoter

SV40 promoter

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf198

Plasmid

#12886

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf198

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf103

Plasmid

#12791

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf103

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Tead4

Plasmid

#128832

Purpose

dox-dependent expression of mouse Tead4

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tead4 (Tead4 Mouse)

Use

Lentiviral

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

Plasmid

#184741

Purpose

encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

Depositor

Randall Platt

Article

Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

Insert

FsRT-Cas1(opt)-Cas2(opt)

Expression

Bacterial

Mutation

sequence codon optimized for expression in E. coli

Promoter

pTetA

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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