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EGFP-Myo1b-Tail

Plasmid

#187365

Purpose

Expresses rat myosin 1b isoform b Tail domain labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

Insert

myosin 1b isoform b Tail domain (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP humanAPOBEC1 L173A/G227A

Plasmid

#167171

Purpose

Expresses human APOBEC1 L173A/G227A in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

humanAPOBEC1 (APOBEC1 Human)

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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gSCPL35-mRFP

Plasmid

#181960

Purpose

SCPL35 protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

SCPL35

Tags

mRFP

Expression

Plant

Promoter

native SCPL35

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/6xHis mCherry F30 Broccoli

Plasmid

#169234

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-Broccoli

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-RhoG-QL-deltaCaaX

Plasmid

#176122

Purpose

Constitutively active, mitochondiral localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Expression

Mammalian

Mutation

QL

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174528

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPRIChp-hOtub1

Plasmid

#174743

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Zhou et al Nat Immunol. 2019 Jul;20(7):879-889. doi

Insert

OTUB1 (OTUB1 Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Nectin-2

Plasmid

#170295

Purpose

A knock-out vector for the mouse Nectin-2

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Nectin-2 gene.

Use

CRISPR and Lentiviral

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Necl-5

Plasmid

#170294

Purpose

A knock-out vector for the mouse Necl-5

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Necl-5 gene.

Use

CRISPR and Lentiviral

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-GgVCL/WT

Plasmid

#162787

Purpose

CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Gallus gallus vinculin (VCL Chicken)

Tags

IRES-GFP

Expression

Bacterial and Mammalian

Mutation

WT

Promoter

beta actin

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjOCT4-Cyan-pac

Plasmid

#160871

Purpose

Marmoset Targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

Insert

mCerulean3

Use

Marmoset targeting

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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OfCasp3b

Plasmid

#166054

Purpose

a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Orbicella faveolata caspase-3b

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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OfCasp3a

Plasmid

#166053

Purpose

a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Orbicella faveolata caspase-3a

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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VTEHDTLLY_epitope

Plasmid

#165017

Purpose

Expression of CMV UL44 (245-253) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (245-253)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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NTTVTFLPTPSLILQ_epitope

Plasmid

#165020

Purpose

Expression of CMV UL44 (37-51) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (37-51)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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SPIC_pet28a

Plasmid

#131654

Purpose

Bacterial expression of ETS gene SPIC

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPIC (SPIC Human)

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlgb

Plasmid

#140293

Purpose

Express soluble His-tagged zebrafish Kitlgb in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-sTomato. PGK-puro

Plasmid

#136264

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

sTomato

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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as-mOPN-PB0

Plasmid

#106450

Purpose

Bacterial expression of antisense to murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

antisense-osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

antisense

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-AM

Plasmid

#153427

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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