We narrowed to 10,136 results for: Coli
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Plasmid#102367PurposeAAV vector to drive TET-ON activator expression from human synapsin I promoterDepositorInsertrtTAV16
UseAAVExpressionMammalianMutationrtTA variant reported in Gene Therapy (2006) 13, …Promoterhuman synapsin I promoterAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
eMscL WT
Plasmid#107454PurposeeMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motifDepositorInsertbacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
UseAdeno-associated viralTagsKir2.1 ER export signal (FCYENEV) and tdTomato fl…ExpressionMammalianPromoterhuman synapsin 1Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Etanercept
Plasmid#182421PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusionDepositorInsertVNp-mNeongreen-Etanercept (TNFRSF1B Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-StefinA
Plasmid#182419PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusionDepositorInsertVNp-mNeongreen-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB009
Plasmid#119707PurposeTranscriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPtrpC::nptII::Ttub
UseSynthetic BiologyAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-BioID-FAM21
Plasmid#121046PurposeExpresses GFP tagged BioID and human FAM21C in mammalian cellsDepositorInsertsTagsGFPExpressionMammalianMutationR118G (highly promiscuous form)PromoterCMVAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T7-6h-GST-α-Synuclein
Plasmid#225224PurposeAlpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.DepositorInsertsGST
α-Synuclein
Tags6xHis Tag and TEV Cleavage SiteExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Lck-TurboID
Plasmid#159433Purposeexpresses Lck-TurboID in mammalian cellsDepositorInsertsTagsHAExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterEF-1aAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK340
Plasmid#192639PurposeExpresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dSpG
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyMutationSoxS has R93A and S101A mutations and dSpG has se…PromoterBBa_J23107 and Sp.pCas9Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only