We narrowed to 15,978 results for: grna

1. TtRMPVIR

   Plasmid

   #27995

   Purpose

   Retroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains DsRed2 and Venus fluorescent reporters.

   Depositor

   Scott Lowe

   Article

   Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

   Insert

   TtRMPVIR (shRen)
   

   Use

   RNAi and Retroviral; Fluorescent reporters

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF280

   Plasmid

   #167675

   Purpose

   Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5aa SunTag VP64 CLV3

   Plasmid

   #115485

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PLKO.1-SMG6-CDS-1

   Plasmid

   #136046

   Purpose

   SMG6 shRNA (Targeting CDS #1) inserted into the PLKO.1 plasmid (AACTTGTAAGTAACCTGCAGC)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   SMG6 (SMG6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PLKO.1-SMG6-CDS-2

   Plasmid

   #136047

   Purpose

   SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   SMG6 (SMG6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5aa SunTag VP64 AP3

   Plasmid

   #115484

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ132-tRNA

   Plasmid

   #179211

   Purpose

   Golden Gate entry vector; 2nd gRNA with tRNA

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   no promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BlatCas9

   Plasmid

   #163792

   Purpose

   The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

   Depositor

   Yongming Wang

   Article

   Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

   Plasmid

   #190794

   Purpose

   Template vector to amplify pieces for creating multiplex sgRNA-arrays.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA-scaffold only
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_J23117

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Level 1 P4 TaU6 guide acceptor

    Plasmid

    #165600

    Purpose

    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 Rheb1 shRNA #1

    Plasmid

    #26625

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pLKO-sh-hSC

    Plasmid

    #46896

    Purpose

    expression of scrambled shRNA

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

    Insert

    scrambled
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLentiCRISPR-v2 sgATG7

    Plasmid

    #211534

    Purpose

    Deletes ATG7

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgATG7
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVTH-sip53

    Plasmid

    #12239

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Insert

    shRNA against p53 (TP53 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSF279

    Plasmid

    #167674

    Purpose

    Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 Rheb1 shRNA #2

    Plasmid

    #26626

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only