We narrowed to 59,318 results for: %s

1. pLenti-U6sgbbBsmbI-puro-2A-Fluc

   Plasmid

   #100277

   Purpose

   Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS1928

   Plasmid

   #205967

   Purpose

   JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH all proteins, with His-tagged Cas8
   

   Tags

   14xHis on Cas8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9 TadCBEc

   Plasmid

   #193834

   Purpose

   Express TadCBEc (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDc-SpCas9 D10A-2xUGI
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS1861

   Plasmid

   #205974

   Purpose

   FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pClneoMyc human CIN85

   Plasmid

   #47935

   Purpose

   expresses Myc tagged human CIN85 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Kawata et al J Biochem. 2006 May;139(5):931-9.

   Insert

   CIN85 (SH3KBP1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   contains amino acids 2-665 of human CIN85; D114G

   Promoter

   CMV

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. VPS13C^halo∆1235-1748

   Plasmid

   #187297

   Purpose

   Express VPS13C truncation mutant in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

   Insert

   VPS13C truncation mutant (VPS13C Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV iGluu

   Plasmid

   #105832

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR S72T variant
   

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Ser 72 to Thr

   Promoter

   CMV

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. cpGFP-Adhiron_flex

   Plasmid

   #165044

   Purpose

   Flexible linker control for POLArISact expression in mammalian cells

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   cpGFP-Adhiron (connected with a flexible linker)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. amilGFP chromoprotein promoter-less

   Plasmid

   #118006

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. KG#203

    Plasmid

    #110880

    Purpose

    Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

    Inserts

    rab-3 promoter
    pde-4d (isoform d) cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

    Plasmid

    #160633

    Purpose

    TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31-RDF
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-HISx6-SIMx6

    Plasmid

    #116938

    Purpose

    Bacterial expression vector for 6 sim

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    6 sim
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-HISx6-SUMOx10

    Plasmid

    #116937

    Purpose

    Bacterial expression vector for 10 sumo

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10 SUMO
    

    Tags

    HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ins:MCS; cryaa:RFP

    Plasmid

    #110282

    Purpose

    A zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

    Depositor

    Nikolay Ninov

    Article

    Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

    Type

    Empty backbone

    Use

    Zebrafish

    Promoter

    insulin

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MO70-N-Flag-hCRACR2A-isoform-a

    Plasmid

    #79592

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

    Insert

    CRACR2A-isoform-a (CRACR2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pQFlag-USP11 CS puroR

    Plasmid

    #46748

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW-ubiquitin-SV40-GFP

    Plasmid

    #65445

    Purpose

    Expression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectors

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    SV40-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBQ and SV40

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

    Plasmid

    #139649

    Purpose

    Reporter assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    H2B-mCherry
    

    Use

    Lentiviral

    Tags

    FLAG, His6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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