We narrowed to 14,749 results for: EGF
-
Plasmid#176118PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP L273D-L276D
Plasmid#114182PurposeORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron-G
Plasmid#36887DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
ExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-T7-EcKlenow (LM2692)
Plasmid#208963PurposeUnfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertEcKlenow-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationEcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pbG-APRT-J-u32uni-PdTKmCh
Plasmid#107279PurposeAPRT c.407T>C (APRT*J) donor vector with unilateral microhomologyDepositorInsertsMutationc.402A>T, c.407T>CAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1:uas-myc-act-map2k2b-2a-tdTomato
Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
PEAR1-bio-His
Plasmid#51860PurposeExpresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPEAR1 (PEAR1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1062
Plasmid#29011PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertPle155-Cre/EGFP-NLS
UseHigh copy number homologous recombination plasmid…ExpressionMammalianPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1424
Plasmid#29220PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertPle155-EGFP-NLS
UseHigh copy number homologous recombination plasmid…ExpressionMammalianPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable SinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAT9752-BEAR-mScarlet
Plasmid#162991PurposeBEAR target plasmid with split mScarlet and disrupted 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta1(617-949)-GFP
Plasmid#100732PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorAvailable SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mCherry-FRB*
Plasmid#207145PurposeLysosome-targeted rapalog dimerizerDepositorInsertLAMP1 (LAMP1 Human)
TagsFRB* (T2098L mutant of FKBP-rapamycin binding dom…ExpressionMammalianMutationSilent mutationPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173874PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
TagsC-terminal CAAX-box and mCherryExpressionMammalianPromoterCMVAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax
Plasmid#173873PurposeMammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorInsertmCherry-NES-SspB-SOS2cat-P2A-iLIDcaax
TagsC-terminal CAAX-box and mCherryExpressionMammalianPromoterCMVAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Phobos
Plasmid#98166Purposeblue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos gene
TagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…PromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7A-mGFP
Plasmid#62633Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
TagsmEGFP inserted in M3-M4 loopExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Anti-LRP4 (cytoplasmic) [N164/6.1R]
Plasmid#114542PurposeMammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.DepositorInsertanti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
ExpressionMammalianPromoterdual CMVAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV uN2C GFP (Ctl)
Plasmid#224355PurposeExpress GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeatsDepositorInsertuN2C
UseAAVTagseGFPExpressionMammalianMutationCodon-optimized for expression in human, so no pu…PromoterCMV + chimeric intyron (CAG)Available SinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGK mScarlet-LC3B
Plasmid#200083PurposeMammalian expression of fluorescently-tagged marker for autophagic vesiclesDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSMT-MjtRNA-GFP(47TAG)
Plasmid#193251PurposeExpresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)DepositorInsertsGFP(47TAG)
MjtRNA
ExpressionBacterialMutationCodon for Tyr47 replaced with amber stop codon TA…Promoterhsp60 and metUAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only